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Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia
- Source :
- AMB Express
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- The lignocellulosic fractions of municipal solid waste (MSW) can be used as renewable resources due to the widespread availability, predictable and low pricing and suitability for most conversion technologies. In particular, after the typical paper recycling loop, the newspaper waste (NW) could be further valorized as feedstock in biorefinering industry since it still contains up to 70 % polysaccharides. In this study, two different physicochemical methods—ammonia fiber expansion (AFEX) and extractive ammonia (EA) were tested for the pretraetment of NW. Furthermore, based on the previously demonstrated ability of the recombinant enzymes endocellulase rCelStrep, α-l-arabinofuranosidase rPoAbf and its evolved variant rPoAbf F435Y/Y446F to improve the saccharification of different lignocellulosic pretreated biomasses (such as corn stover and Arundo donax), in this study these enzymes were tested for the hydrolysis of pretreated NW, with the aim of valorizing the lignocellulosic fractions of the MSW. In particular, a mixture of purified enzymes containing cellulases, xylanases and accessory hemicellulases, was chosen as reference mix and rCelStrep and rPoAbf or its variant were replaced to EGI and Larb. The results showed that these enzymatic mixes are not suitable for the hydrolysis of NW after AFEX or EA pretreatment. On the other hand, when the enzymes rCelStrep, rPoAbf and rPoAbf F435Y/Y446F were tested for their effect in hydrolysis of pretreated NW by addition to a commercial enzyme mixture, it was shown that the total polysaccharides conversion yield reached 37.32 % for AFEX pretreated NW by adding rPoAbf to the mix whilst the maximum sugars conversion yield for EA pretreated NW was achieved 40.80 % by adding rCelStrep. The maximum glucan conversion yield obtained (45.61 % for EA pretreated NW by adding rCelStrep to the commercial mix) is higher than or comparable to those reported in recent manuscripts adopting hydrolysis conditions similar to those used in this study.
- Subjects :
- 0106 biological sciences
020209 energy
Biophysics
02 engineering and technology
Cellulase
Raw material
7. Clean energy
01 natural sciences
Applied Microbiology and Biotechnology
Newspaper waste
Hydrolysis
Ammonia
chemistry.chemical_compound
Arabinofuranosidase
Hemicellulase
010608 biotechnology
EA pretreatment
0202 electrical engineering, electronic engineering, information engineering
Biorefining
biology
business.industry
Municipal solid waste
AFEX pretreatment
Pulp and paper industry
Biotechnology
Paper recycling
Corn stover
Biophysic
chemistry
Yield (chemistry)
biology.protein
Original Article
business
Subjects
Details
- ISSN :
- 21910855
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- AMB Express
- Accession number :
- edsair.doi.dedup.....74172f5d59c6e405207e2fbc89e7e73a