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Molecular detection of multidrug-resistant Mycobacterium leprae from Indian leprosy patients
- Source :
- Journal of global antimicrobial resistance. 12
- Publication Year :
- 2017
-
Abstract
- Objectives The emergence of multidrug-resistant (MDR) organisms for any infectious disease is a public health concern. Global efforts to control leprosy by intensive chemotherapy have led to a significant decrease in the number of registered patients. Currently recommended control measures for treating leprosy with multidrug therapy (MDT) were designed to prevent the spread of dapsone-resistant Mycobacterium leprae strains. Here we report the identification of MDR M. leprae from relapse leprosy patients from endemic regions in India. Methods Resistance profiles to rifampicin, dapsone and ofloxacin of the isolated strains were confirmed by identification of mutations in genes previously shown to be associated with resistance to each drug. Between 2009–2016, slit-skin smear samples were collected from 239 relapse and 11 new leprosy cases from hospitals of The Leprosy Mission across India. DNA was extracted from the samples and was analysed by PCR targeting the rpoB, folP and gyrA genes associated with resistance to rifampicin, dapsone and ofloxacin, respectively, in M. leprae. M. leprae Thai-53 (wild-type) and Zensho-4 (MDR) were used as reference strains. Results Fifteen strains showed representative mutations in at least two resistance genes. Two strains showed mutations in all three genes responsible for drug resistance. Seven, seven and one strain, respectively, showed mutations in genes responsible for rifampicin and dapsone resistance, for dapsone and ofloxacin resistance and for rifampicin and ofloxacin resistance. Conclusion This study showed the emergence of MDR M. leprae in MDT-treated leprosy patients from endemic regions of India.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Adult
Male
Adolescent
030231 tropical medicine
030106 microbiology
Immunology
India
Leprostatic Agents
Drug resistance
Microbial Sensitivity Tests
Dapsone
Microbiology
03 medical and health sciences
Young Adult
0302 clinical medicine
Leprosy
Drug Resistance, Bacterial
medicine
Immunology and Allergy
Humans
Mycobacterium leprae
Aged
biology
business.industry
Middle Aged
rpoB
biology.organism_classification
medicine.disease
Multiple drug resistance
Female
Ofloxacin
business
Rifampicin
medicine.drug
Subjects
Details
- ISSN :
- 22137173
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of global antimicrobial resistance
- Accession number :
- edsair.doi.dedup.....741ffcfd624ee79bb8f12558056ccd1c