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Anti-Inflammatory Activity of Some Characteristic Constituents from the Vine Stems of Spatholobus suberectus
- Source :
- Molecules, Vol 24, Iss 20, p 3750 (2019), Molecules, Volume 24, Issue 20
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- The dried vine stems of Spatholobus suberectus are commonly used in traditional Chinese medicine for treating gynecological and cardiovascular diseases. In this study, five new compounds named spasuberol A (2), homovanillyl-4-oxo-nonanoate (5), spasuberol C (6), spasuberoside A (14), and spasuberoside B (15), together with ten known compounds (1, 3, 4, 7&ndash<br />13), were isolated from the dried vine stems of S. suberectus. Their chemical structures were analyzed using spectroscopic assays. This is the first study interpreting the detailed structural information of 4. The anti-inflammatory activity of these compounds was evaluated by reducing nitric oxide overproduction in RAW264.7 macrophages stimulated by lipopolysaccharide. Compounds 1 and 8&ndash<br />10 showed strong inhibitory activity with half maximal inhibitory concentration (IC50) values of 5.69, 16.34, 16.87, and 6.78 &mu<br />M, respectively, exhibiting higher activity than the positive drug l-N6-(1-iminoethyl)-lysine (l-NIL) with an IC50 value of 19.08 &mu<br />M. The IC50 values of inhibitory activity of compounds 2 and 4&ndash<br />6 were 46.26, 40.05, 45.87, and 28.29 &mu<br />M respectively, which were lower than l-NIL, but better than that of positive drug indomethacin with an IC50 value of 55.44 &mu<br />M. Quantitative real-time polymerase chain reaction analysis revealed that assayed compounds with good anti-inflammatory activity, such as 1, 6, 9, and 10 at different concentrations, can reduce the messenger RNA (mRNA) expression of some pro-inflammatory cytokines such as tumor necrosis factor &alpha<br />(TNF-&alpha<br />), nitric oxide synthase (iNOS), and cyclooxygenase 2 (COX-2). The anti-inflammatory activity and the possible mechanism of the compounds mentioned in this paper were studied preliminarily.
- Subjects :
- Lipopolysaccharides
Lipopolysaccharide
Indomethacin
Anti-Inflammatory Agents
Gene Expression
Nitric Oxide Synthase Type II
Pharmaceutical Science
Pharmacology
Analytical Chemistry
Mice
chemistry.chemical_compound
0302 clinical medicine
Drug Discovery
Glycosides
Medicine, Chinese Traditional
isoflavones
anti-inflammatory activity
0303 health sciences
Plant Stems
biology
Fabaceae
Isoflavones
spatholobus suberectus
Nitric oxide synthase
Spatholobus suberectus
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Molecular Medicine
medicine.drug_class
Nitric Oxide
raw264.7 macrophage cells
Article
Anti-inflammatory
Nitric oxide
lcsh:QD241-441
Inhibitory Concentration 50
03 medical and health sciences
lcsh:Organic chemistry
medicine
Animals
Humans
Physical and Theoretical Chemistry
IC50
030304 developmental biology
Messenger RNA
Plant Extracts
Tumor Necrosis Factor-alpha
Lysine
Organic Chemistry
inhibition of nitric oxide production
RAW 264.7 Cells
chemistry
Cyclooxygenase 2
biology.protein
Cyclooxygenase
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 24
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....74433fe4cf707a9c04b49b1a18dccfc4