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Validation of a new immunofluorescence antibody test for the detection of Leishmania infantum infection in cats
- Source :
- Parasitology research. 119(4)
- Publication Year :
- 2019
-
Abstract
- The prevalence data of Leishmania infantum infection in cats are characterized by a large variability mainly attributed to the differences in diagnostic techniques. In the absence of consensus about the method of choice for diagnosing feline leishmaniosis, the performance of a new immunofluorescence antibody test (IFAT) was herein analytically described by the comparison with IFAT commonly used for the diagnosis of canine leishmaniosis (i.e., IFAT-OIE) and a laboratory enzyme-linked immunosorbent assay (ELISA). Sera of cats living in visceral leishmaniosis–endemic (n = 105) and visceral leishmaniosis–non-endemic (n = 50) areas were tested by the above methodologies and real-time PCR (qPCR). The most frequent result was represented by triple negativity to the three tests (IFAT-OIE, ELISA, and qPCR) in 42.9% and 80% cats from endemic and non-endemic areas, respectively. Bayes latent class analysis gave an output probability of 34.1% (posterior standard deviation, psd = 5.4%) of true L. infantum cases (TCL) which represent the true estimated prevalence of infection. The sensitivity of each variable contributing to define the TCL was 24% (psd = 6.3%) for qPCR, 78.8% (psd = 8.7%) for ELISA and 91.8% (psd = 5.2%) for IFAT-OIE. The probability to be a TCL was 94.5% for the sample from an endemic area. The cross-validation of the new IFAT by a logistic model correctly identified as positive 80.7% of subjects defined as TCL and negative 89.9% as not TCL, respectively, by the Bayesian model. The study results estimate a good accuracy of the IFAT in predicting cats exposed to L. infantum. Therefore, this procedure may be beneficial for screening cat populations for a better understanding of the epidemiology of feline leishmaniosis.
- Subjects :
- Male
medicine.medical_specialty
Antibodies, Protozoan
Enzyme-Linked Immunosorbent Assay
Immunofluorescence
Logistic regression
Cat Diseases
Real-Time Polymerase Chain Reaction
Medical microbiology
parasitic diseases
medicine
Animals
Leishmania infantum
CATS
General Veterinary
medicine.diagnostic_test
biology
Endemic area
Negativity effect
Bayes Theorem
General Medicine
biology.organism_classification
Virology
Infectious Diseases
Fluorescent Antibody Technique, Direct
Insect Science
biology.protein
Cats
Leishmaniasis, Visceral
Parasitology
Antibody
Subjects
Details
- ISSN :
- 14321955
- Volume :
- 119
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Parasitology research
- Accession number :
- edsair.doi.dedup.....747bf85be596b974159335729c457dce