Back to Search
Start Over
Assessment of reproducibility and biological variability of fasting and postprandial plasma metabolite concentrations using 1H NMR spectroscopy
- Source :
- PLoS ONE, Vol 14, Iss 6, p e0218549 (2019), PLoS ONE, PLOS ONE, 14(6):e0218549. PUBLIC LIBRARY SCIENCE, Li-Gao, R, Hughes, D A, le Cessie, S, de Mutsert, R, den Heijer, M, Rosendaal, F R, van Dijk, K W, Timpson, N J & Mook-Kanamori, D O 2019, ' Assessment of reproducibility and biological variability of fasting and postprandial plasma metabolite concentrations using 1H NMR spectroscopy ', PLoS ONE, vol. 14, no. 6, e0218549 . https://doi.org/10.1371/journal.pone.0218549, PLoS ONE, 14(6), PLoS ONE, 14(6):e0218549. Public Library of Science, Li-Gao, R, Hughes, D A, le Cessie, S, de Mutsert, R, den Heijer, M, Rosendaal, F R, Willems van Dijk, K, Timpson, N J & Mook-Kanamori, D O 2019, ' Assessment of reproducibility and biological variability of fasting and postprandial plasma metabolite concentrations using 1 H NMR spectroscopy ', PLoS ONE, vol. 14, no. 6, e0218549 . https://doi.org/10.1371/journal.pone.0218549
- Publication Year :
- 2019
-
Abstract
- INTRODUCTION: It is crucial to understand the factors that introduce variability before applying metabolomics to clinical and biomarker research.OBJECTIVES: We quantified technical and biological variability of both fasting and postprandial metabolite concentrations measured using 1H NMR spectroscopy in plasma samples.METHODS: In the Netherlands Epidemiology of Obesity study (n = 6,671), 148 metabolite concentrations (101 metabolites belonging to lipoprotein subclasses) were measured under fasting and postprandial states (150 minutes after a mixed liquid meal). Technical variability was evaluated among 265 fasting and 851 postprandial samples, with the identical blood plasma sample being measured twice by the same laboratory protocol. Biological reproducibility was assessed by measuring 165 individuals twice across time for evaluation of short- (3 years) biological variability. Intra-class coefficients (ICCs) were used to assess variability. The ICCs of the fasting metabolites were compared with the postprandial metabolites using two-sided paired Wilcoxon test separately for short- and long-term measurements.RESULTS: Both fasting and postprandial metabolite concentrations showed high technical reproducibility using 1H NMR spectroscopy (median ICC = 0.99). Postprandial metabolite concentrations revealed slightly higher ICC scores than fasting ones in short-term repeat measures (median ICC in postprandial and fasting metabolite concentrations 0.72 versus 0.67, Wilcoxon p-value = 8.0×10-14). Variability did not increase further in a long-term repeat measure, with median ICC in postprandial of 0.64 and in fasting metabolite concentrations 0.66.CONCLUSION: Technical reproducibility is excellent. Biological reproducibility of postprandial metabolite concentrations showed a less or equal variability than fasting metabolite concentrations over time.
- Subjects :
- 0301 basic medicine
Male
Magnetic Resonance Spectroscopy
Physiology
Metabolite
Spectrum analysis techniques
Biochemistry
chemistry.chemical_compound
0302 clinical medicine
Glucose Metabolism
Blood plasma
Metabolites
Medicine and Health Sciences
EPIDEMIOLOGY
education.field_of_study
Multidisciplinary
Fatty Acids
Fasting
Research Assessment
Middle Aged
Postprandial Period
Lipids
Reproducibility
Body Fluids
Postprandial
Blood
030220 oncology & carcinogenesis
Metabolome
Carbohydrate Metabolism
Medicine
Female
Analysis of variance
Anatomy
Bristol Population Health Science Institute
Research Article
medicine.medical_specialty
Science
Population
METABOLOMICS
Blood Plasma
03 medical and health sciences
Metabolomics
NMR spectroscopy
Internal medicine
medicine
Humans
education
Analysis of Variance
Biology and Life Sciences
Reproducibility of Results
Research and analysis methods
030104 developmental biology
Endocrinology
Metabolism
chemistry
Biological Variation, Population
Biomarkers
Blood Chemical Analysis
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....748a7c7d5e35c6c66bfe4c1aaa02413c