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Double Flow Bioreactor for In Vitro Test of Drug Delivery
- Source :
- Current drug delivery. 14(2)
- Publication Year :
- 2015
-
Abstract
- In this work, double-structured polymeric scaffolds were produced, and a double flow bioreactor was designed and set up in order to create a novel system to carry out advanced in vitro drug delivery tests. The scaffolds, consisting of a cylindrical porous matrix, are able to host cells, thus mimicking a three-dimensional tumor mass: moreover, a âpseudo-vascularâ structure was embedded into the matrix, with the aim of allowing a flow circulation. The structure that emulates a blood vessel is a porous tubular-shaped scaffold prepared by Diffusion Induced Phase Separation (DIPS), with an internal lumen of 2 mm and a wall thickness of 200 micrometers. The as-prepared vessel was incorporated into a three-dimensional matrix, prepared by Thermally Induced Phase Separation (TIPS), characterized by a high porosity (about 95%) and pore size adequate to accommodate tumor cells and/or mesenchymal cells. The morphology of the multifunctional scaffolds is easy-tunable in terms of pore size, porosity and thickness and therefore adaptable to various cell or tissue types. At the same time, a double flow bioreactor was designed and built up, in order to be able to carry out biological tests on the scaffold under dynamic conditions. The device allows a separate control of the two flows (one for the tubular scaffold, one for the porous matrix) through the scaffolds. Preliminary characterizations and tests carried out suggest the presented system as a candidate to suitably âin vitroâ assess the effects of different drugs on various cell populations.
- Subjects :
- Scaffold
Materials science
In vitro test
Pharmaceutical Preparation
Polymers
Surface Properties
Surface Propertie
Bioreactor
Phase separation
Drug Evaluation, Preclinical
Vascular tissue engineering
Pharmaceutical Science
Nanotechnology
02 engineering and technology
010402 general chemistry
01 natural sciences
Fluid dynamic
Bioreactors
Drug Delivery Systems
Humans
Particle Size
Polymer
Porosity
chemistry.chemical_classification
Fluid dynamic, Vascular Tissue Engineering
Medicine (all)
Equipment Design
021001 nanoscience & nanotechnology
0104 chemical sciences
Shear stre
chemistry
Pharmaceutical Preparations
Poly-L-lactic acid
Drug delivery
Particle size
0210 nano-technology
Drug Delivery System
Human
Lumen (unit)
Biomedical engineering
Subjects
Details
- ISSN :
- 18755704
- Volume :
- 14
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Current drug delivery
- Accession number :
- edsair.doi.dedup.....74c8a079a5ba7dc7e9d0e3ce82a8f517