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Measurement of Indoleamine 2, 3 Dioxygenase Activity in Colorectal Cancer: A Controlled Group Study
- Source :
- Journal of Clinical and Cellular Immunology, Journal of Clinical and Cellular Immunology, OMICS Publishing Group, 2015, 06 (06), pp.1000374. ⟨10.4172/2155-9899.1000374⟩
- Publication Year :
- 2015
- Publisher :
- HAL CCSD, 2015.
-
Abstract
- International audience; Background: Indoleamine 2,3-dioxygenase (IDO), the rate-limiting enzyme that converts tryptophan (Trp) to kynurenine (Kyn), suppresses antitumor immune responses via depletion of Trp and accumulation of Kyn. We hypothesized that, in colorectal cancer (CRC), IDO activity may serve as a biomarker and thus we compared the IDO activity between patients with CRC and those without CRC. We further assessed the effect of surgical treatment of CRC on IDO activity.Methods: Serum concentrations of Trp and Kyn were measured by high performance liquid chromatography in the sera of 68 patients with CRC (CRC group) and 38 without CRC (Control group) prior surgery (D0) and 7 days after surgery (D7). The IDO activity was estimated by the serum Kyn-to-Trp ratio (Kyn/Trp ratio).Results: At Day 0, serum Kyn concentration was higher in the CRC group than in Control group (1.7 [1.4;2.1] μM vs 1.25 [0.9;1.78] μM, respectively; p=0.004) while no difference in serum concentration of Trp was observed between the two groups. Kyn/Trp ratio (IDO activity) was significantly higher in the CRC group than in Control group. At Day 7 serum concentrations of Trp, Kyn and the Kyn/Trp ratio were not statistically different between the two groups.Conclusion: This study indicates that IDO activity is higher in patients with CRC compared with those without CRC. Surgical treatment impacts the IDO activity with a similar Kyn/Trp ratio in both groups. This study is the first step to larger studies to establish the Kyn/Trp ratio as a reliable serum marker of CRC.
- Subjects :
- medicine.medical_specialty
Colorectal cancer
[SDV.CAN]Life Sciences [q-bio]/Cancer
chemistry.chemical_compound
Immune system
Internal medicine
medicine
Serum concentration
Indoleamine 2,3-dioxygenase
chemistry.chemical_classification
business.industry
Kynurénine
Tryptophan
Biomarker
medicine.disease
digestive system diseases
3. Good health
Enzyme
Endocrinology
chemistry
Indoleamine 2,3
Immunology
dioxygenase activity
Biomarker (medicine)
[SDV.IMM]Life Sciences [q-bio]/Immunology
business
Kynurenine
Subjects
Details
- Language :
- English
- ISSN :
- 21559899
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical and Cellular Immunology, Journal of Clinical and Cellular Immunology, OMICS Publishing Group, 2015, 06 (06), pp.1000374. ⟨10.4172/2155-9899.1000374⟩
- Accession number :
- edsair.doi.dedup.....74d0a19b5fc833b0b4b15950742cc2bb