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Non-invasive tracking of CD4+ T cells with a paramagnetic and fluorescent nanoparticle in brain ischemia
- Source :
- Journal of Cerebral Blood Flow & Metabolism
- Publication Year :
- 2015
-
Abstract
- Recent studies have demonstrated that lymphocytes play a key role in ischemic brain injury. However, there is still a lack of viable approaches to non-invasively track infiltrating lymphocytes and reveal their key spatiotemporal events in the inflamed central nervous system (CNS). Here we describe an in vivo imaging approach for sequential monitoring of brain-infiltrating CD4+ T cells in experimental ischemic stroke. We show that magnetic resonance imaging (MRI) or Xenogen imaging combined with labeling of SPIO-Molday ION Rhodamine-B (MIRB) can be used to monitor the dynamics of CD4+ T cells in a passive transfer model. MIRB-labeled CD4+ T cells can be longitudinally visualized in the mouse brain and peripheral organs such as the spleen and liver after cerebral ischemia. Immunostaining of tissue sections showed similar kinetics of MIRB-labeled CD4+ T cells when compared with in vivo observations. Our results demonstrated the use of MIRB coupled with in vivo imaging as a valid method to track CD4+ T cells in ischemic brain injury. This approach will facilitate future investigations to identify the dynamics and key spatiotemporal events for brain-infiltrating lymphocytes in CNS inflammatory diseases.
- Subjects :
- 0301 basic medicine
CD4-Positive T-Lymphocytes
Male
lymphocytes
Pathology
medicine.medical_specialty
Central nervous system
Ischemia
Spleen
Biology
Fluorescence
Brain Ischemia
Brain ischemia
03 medical and health sciences
0302 clinical medicine
In vivo
medicine
Animals
Magnetite Nanoparticles
Fluorescent Dyes
Mice, Knockout
Ischemic stroke
medicine.diagnostic_test
Staining and Labeling
Rhodamines
Magnetic resonance imaging
Original Articles
medicine.disease
Magnetic Resonance Imaging
USPIO
CD4+ T cells
3. Good health
DNA-Binding Proteins
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Neurology
Cell Tracking
In vivo imaging
Neurology (clinical)
Cardiology and Cardiovascular Medicine
030217 neurology & neurosurgery
Preclinical imaging
Immunostaining
Subjects
Details
- ISSN :
- 15597016
- Volume :
- 36
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
- Accession number :
- edsair.doi.dedup.....74f01d4a247ba9c433bd92a4649785ab