Back to Search
Start Over
Transcriptome analysis reveals the effects of alkali stress on root system architecture and endogenous hormones in apple rootstocks
- Source :
- Journal of Integrative Agriculture, Vol 18, Iss 10, Pp 2264-2271 (2019)
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Soil alkalinity is a major factor that restricts the growth of apple roots. To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia (alkali-tolerant) and Malus hupehensis (alkali-sensitive), were compared. To understand alkali tolerance of M. prunifolia at the molecular level, transcriptome analysis was performed. When plants were cultured in alkaline conditions for 15 d, the root growth of M. hupehensis with weak alkali tolerance decreased significantly. Analysis of endogenous hormone levels showed that the concentrations of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in M. hupehensis under alkali stress were lower than those in the control. However, the trend for IAA and ZR in M. prunifolia was the opposite. The concentration of abscisic acid (ABA) in the roots of the two apple rootstocks under alkali stress increased, but the concentration of ABA in the roots of M. prunifolia was higher than that in M. hupehensis. The expression of IAA-related genes ARF5, GH3.6, SAUR36, and SAUR32 and the Cytokinin (CTK)-related gene IPT5 in M. prunifolia was higher than those in the control, but the expression of these genes in M. hupehensis was lower than those in the control. The expression of ABA-related genes CIPK1 and AHK1 increased in the two apple rootstocks under alkali stress, but the expression of CIPK1 and AHK1 in M. prunifolia was higher than in M. hupehensis. These results demonstrated that under alkali stress, the increase of IAA, ZR, and ABA in roots and the increase of the expression of related genes promoted the growth of roots and improved the alkali tolerance of apple rootstocks.
- Subjects :
- 0106 biological sciences
Agriculture (General)
Plant Science
Root system
root architecture
01 natural sciences
Biochemistry
endogenous hormone
S1-972
Transcriptome
chemistry.chemical_compound
Alkali soil
transcriptome analysis
Food Animals
Malus prunifolia
Malus hupehensis
Abscisic acid
Ecology
biology
Chemistry
fungi
food and beverages
04 agricultural and veterinary sciences
biology.organism_classification
apple rootstock
Horticulture
Cytokinin
040103 agronomy & agriculture
0401 agriculture, forestry, and fisheries
Animal Science and Zoology
alkali stress
Rootstock
Agronomy and Crop Science
010606 plant biology & botany
Food Science
Subjects
Details
- ISSN :
- 20953119
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Agriculture
- Accession number :
- edsair.doi.dedup.....74ff896cf9a27f54f6632b680e73fea8