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Divergent immune roles of two fucolectin isoforms in Apostichopus japonicus
- Source :
- Developmental and comparative immunology. 89
- Publication Year :
- 2018
-
Abstract
- The F-type lectin (fucolectin) family represents a new group with innate immunity. In this study, two fucolectin isoforms (designated as AjFTL-1 and AjFTL-2) were identified in sea cucumber (Apostichopus japonicus) through rapid amplification of cDNA ends. Full-length cDNAs of AjFTL-1 and AjFTL-2 measured 2134 and 1286 bp, encoding two secreted proteins comprising 317 and 181 amino acid residues, respectively. The signal peptide, l-fucose binding motif ("HX(26)RXDX(4)R/K") and cation binding sequence motif ("h2DGx") were conserved in AjFTL-1 and AjFTL-2. However, AjFTL-1 contains an additional complement control protein domain. Multiple sequence alignments supported that AjFTL-1 and AjFTL-2 are new members of the F-type lectin family. Tissues distribution analysis indicated that both AjFTL-1 and AjFTL-2 were widely expressed in all tested tissues, featuring differential expression patterns. Vibrio splendidus infection in vivo can significantly upregulate the mRNA transcripts of the two genes, with a larger magnitude observed in AjFTL-1. By contrast, lipopolysaccharide stimulation in vitro can markedly induce the expression level of AjFTL-2 but not that of AjFTL-1. Silencing AjFTL-2 by siRNA can suppress the AjNOS transcript, whereas injection of the recombinant protein of AjFTL-2 can significantly induce AjNOS expression. By contrast, the loss- and gain-of-function of AjFTL-1 caused no effect on the expression of AjNOS. Our present study provides evidence supporting that AjFTL-1 and AjFTL-2 play diverse roles in the innate immune defense of sea cucumbers toward bacterial infection.
- Subjects :
- 0301 basic medicine
Signal peptide
Cation binding
DNA, Complementary
Immunology
Gene Expression
Biology
03 medical and health sciences
Rapid amplification of cDNA ends
Lectins
Gene expression
Animals
Protein Isoforms
Tissue Distribution
RNA, Messenger
Cloning, Molecular
Vibrio
Innate immune system
030102 biochemistry & molecular biology
biology.organism_classification
Immunity, Innate
Cell biology
030104 developmental biology
Stichopus
Apostichopus japonicus
biology.protein
Sequence motif
Developmental Biology
Complement control protein
Subjects
Details
- ISSN :
- 18790089
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Developmental and comparative immunology
- Accession number :
- edsair.doi.dedup.....756741ed8bf8d03468f649b13aff3fbb