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UbcH10 expression on thyroid fine-needle aspirates
- Source :
- Cancer cytopathology 118 (2010): 157–165. doi:10.1002/cncy.20046, info:cnr-pdr/source/autori:Guerriero, Eliana; Ferraro, Angelo; Desiderio, Doriana; Pallante, Pierlorenzo; Berlingieri, Maria Teresa; Iaccarino, Antonino; Palmieri, Emiliano; Palombini, Lucio; Fusco, Alfredo; Troncone, Giancarlo/titolo:UbcH10 Expression on Thyroid Fine-Needle Aspirates/doi:10.1002%2Fcncy.20046/rivista:Cancer cytopathology/anno:2010/pagina_da:157/pagina_a:165/intervallo_pagine:157–165/volume:118
- Publication Year :
- 2010
- Publisher :
- Wiley, 2010.
-
Abstract
- BACKGROUND: Thyroid fine-needle aspiration (FNA) samples belonging to the follicular neoplasm/suspicious for malignancy classes are controversial. The authors identified UbcH10 as a marker useful in the diagnosis of several neoplasms, including thyroid cancer. Here, analysis of UbcH10 expression by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry was applied to FNAs. METHODS: A series of 84 follicular neoplasm/suspicious for malignancy FNAs with histological follow-up (30 malignant) was prospectively collected. UbcH10 immunostaining was performed on cell blocks and compared with that of the proliferation marker Ki-67. At the mRNA level, UbcH10 was compared with CCND2 and PCSK2 expression, these latter being the best performing components of the previously reported 3-gene assay; to determine the diagnostic accuracy, the area under the curve (AUC) of the receiver operating characteristic (ROC) curve for each gene individually and in combination was evaluated. RESULTS: UbcH10 and Ki-67 shared a similar pattern; although UbcH10 expression was higher in malignant than in benign lesions (P < .001), staining was sporadic, and the cutoff value derived by the ROC analysis was too low (1.25%) for routine application. Conversely, UbcH10 expression assessment by quantitative RT-PCR was effective. UbcH10 mRNA levels associated with malignant histology were significantly higher than those associated with benign histology (P = .02). The AUC was 0.74 for UbcH10, 0.81 for CCDN2, 0.62 for PCSK2, and 0.84 for UbcH10 and CCND2 combination. CONCLUSIONS: UbcH10 quantitative RT-PCR analysis, rather than immunohistochemistry, is useful to increase the detection of malignancy in thyroid FNAs. UbcH10 may be added as a panel component in quantitative RT-PCR–based assays. Cancer (Cancer Cytopathol) 2010. © 2010 American Cancer Society.
- Subjects :
- Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Adolescent
Biopsy, Fine-Needle
Thyroid Gland
Malignancy
thyroid
Biopsy
Biomarkers, Tumor
Humans
Medicine
fine-needle aspiration
RNA, Messenger
Thyroid Neoplasms
Thyroid cancer
Aged
Suspicious for Malignancy
medicine.diagnostic_test
biology
Reverse Transcriptase Polymerase Chain Reaction
business.industry
3-gene assay
Thyroid
Cancer
Middle Aged
UbcH10
medicine.disease
Immunohistochemistry
Ki-67 Antigen
Fine-needle aspiration
medicine.anatomical_structure
Oncology
Ki-67
Ubiquitin-Conjugating Enzymes
biology.protein
Female
business
Subjects
Details
- ISSN :
- 19346638 and 1934662X
- Volume :
- 118
- Database :
- OpenAIRE
- Journal :
- Cancer Cytopathology
- Accession number :
- edsair.doi.dedup.....756801dc0bbbafb3e0640f8630558b2b