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Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29
- Source :
- Analytical Cellular Pathology, Vol 2021 (2021), Analytical Cellular Pathology (Amsterdam)
- Publication Year :
- 2021
- Publisher :
- Hindawi Limited, 2021.
-
Abstract
- Interleukin 10 (IL-10) is a synthetic inhibitor of human cytokines with immunomodulatory and anti-inflammatory effects. This study was designed to investigate the expression variation of IL-10 in the multiple sites including cortex, hippocampus, and lung tissues of neonatal hypoxic-ischemic (HI) rats and explore the crucial role of IL-10 in alleviating HI brain damage. In this study, neonatal Sprague-Dawley rats were subjected to the right common carotid artery ligation, followed by 2 h of hypoxia. The expression of IL-10 in the cortex, hippocampus, and lung tissues was measured with immunohistochemistry, real-time quantitative polymerase chain reaction (RT-qPCR), and western blot (WB). Immunofluorescence double staining was performed to observe the localization of IL-10 in neurons and astrocytes. Moreover, not-targeting and targeting IL-10 siRNA lentivirus vectors were injected into the rats of the negative control (NC) and IL-10 group, respectively, and the mRNA levels of B-cell lymphoma 2 (Bcl-2) and endoplasmic reticulum protein 29 (ERp29) were detected by RT-qPCR following IL-10 silence. The results demonstrated that the IL-10 expression was markedly increased after HI and IL-10 were colocalized with neurons and astrocytes which were badly injured by HI insult. In addition, Bcl-2 and ERp29 were remarkably decreased following IL-10 mRNA interference compared with the NC group. Our findings revealed that IL-10 exerted its antiapoptotic and neuroprotective effects by regulating the expression of Bcl-2 and ERp29, indicating that IL-10 may be a promising molecule target for HIE treatment. Refereed/Peer-reviewed
- Subjects :
- 0301 basic medicine
Cancer Research
Article Subject
Blotting, Western
lung tissues
Hippocampus
Neuroprotection
Pathology and Forensic Medicine
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
carotid artery ligation
Western blot
medicine
Animals
Humans
Lung
Heat-Shock Proteins
RC254-282
hypoxic-ischemic (HI)
Cerebral Cortex
Messenger RNA
QH573-671
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
Chemistry
human cytokines
Endoplasmic reticulum
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Cell Biology
General Medicine
Molecular biology
Interleukin-10
rats
Disease Models, Animal
Interleukin 10
030104 developmental biology
Real-time polymerase chain reaction
Animals, Newborn
Gene Expression Regulation
Proto-Oncogene Proteins c-bcl-2
Hypoxia-Ischemia, Brain
Molecular Medicine
Immunohistochemistry
Cytology
030217 neurology & neurosurgery
Research Article
Subjects
Details
- ISSN :
- 22107185 and 22107177
- Volume :
- 2021
- Database :
- OpenAIRE
- Journal :
- Analytical Cellular Pathology
- Accession number :
- edsair.doi.dedup.....75776ba098d8da5c0932b4580decbd37