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Comparative metatranscriptomics reveals extracellular electron transfer pathways conferring microbial adaptivity to surface redox potential changes
- Source :
- The ISME Journal
- Publication Year :
- 2018
- Publisher :
- Nature Publishing Group UK, 2018.
-
Abstract
- Some microbes can capture energy through redox reactions with electron flow to solid-phase electron acceptors, such as metal-oxides or poised electrodes, via extracellular electron transfer (EET). While diverse oxide minerals, exhibiting different surface redox potentials, are widely distributed on Earth, little is known about how microbes sense and use the minerals. Here we show electrochemical, metabolic, and transcriptional responses of EET-active microbial communities established on poised electrodes to changes in the surface redox potentials (as electron acceptors) and surrounding substrates (as electron donors). Combination of genome-centric stimulus-induced metatranscriptomics and metabolic pathway investigation revealed that nine Geobacter/Pelobacter microbes performed EET activity differently according to their preferable surface potentials and substrates. While the Geobacter/Pelobacter microbes coded numerous numbers of multi-heme c-type cytochromes and conductive e-pili, wide variations in gene expression were seen in response to altering surrounding substrates and surface potentials, accelerating EET via poised electrode or limiting EET via an open circuit system. These flexible responses suggest that a wide variety of EET-active microbes utilizing diverse EET mechanisms may work together to provide such EET-active communities with an impressive ability to handle major changes in surface potential and carbon source availability.
- Subjects :
- 0301 basic medicine
030106 microbiology
Cytochrome c Group
Heme
Biology
Electrochemistry
Microbiology
Redox
Article
Electron Transport
03 medical and health sciences
Electron transfer
Extracellular
Electrodes
Ecology, Evolution, Behavior and Systematics
Pelobacter
chemistry.chemical_classification
Microbiota
Electric Conductivity
Electron acceptor
biology.organism_classification
Carbon
Metabolic pathway
030104 developmental biology
chemistry
Biophysics
cardiovascular system
lipids (amino acids, peptides, and proteins)
Metagenomics
Geobacter
Transcriptome
Oxidation-Reduction
Metabolic Networks and Pathways
Subjects
Details
- Language :
- English
- ISSN :
- 17517370 and 17517362
- Volume :
- 12
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- The ISME Journal
- Accession number :
- edsair.doi.dedup.....75a2456263bf86495d532b8bd3204657