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Evaluation of chronic lymphocytic leukemia by oligonucleotide-based microarray analysis uncovers novel aberrations not detected by FISH or cytogenetic analysis
- Source :
- Molecular Cytogenetics, Molecular Cytogenetics, Vol 4, Iss 1, p 25 (2011)
- Publication Year :
- 2011
- Publisher :
- BioMed Central, 2011.
-
Abstract
- Background Cytogenetic evaluation is a key component of the diagnosis and prognosis of chronic lymphocytic leukemia (CLL). We performed oligonucleotide-based comparative genomic hybridization microarray analysis on 34 samples with CLL and known abnormal karyotypes previously determined by cytogenetics and/or fluorescence in situ hybridization (FISH). Results Using a custom designed microarray that targets >1800 genes involved in hematologic disease and other malignancies, we identified additional cryptic aberrations and novel findings in 59% of cases. These included gains and losses of genes associated with cell cycle regulation, apoptosis and susceptibility loci on 3p21.31, 5q35.2q35.3, 10q23.31q23.33, 11q22.3, and 22q11.23. Conclusions Our results show that microarray analysis will detect known aberrations, including microscopic and cryptic alterations. In addition, novel genomic changes will be uncovered that may become important prognostic predictors or treatment targets for CLL in the future.
- Subjects :
- oligonucleotide
medicine.medical_specialty
Microarray
lcsh:QH426-470
Chronic lymphocytic leukemia
Biology
Chromosome aberration
Biochemistry
cytogenetics
FISH
medicine
Genetics
Genetics(clinical)
Molecular Biology
Genetics (clinical)
Biochemistry, medical
medicine.diagnostic_test
Microarray analysis techniques
Research
Biochemistry (medical)
Cytogenetics
medicine.disease
Human genetics
lcsh:Genetics
Cancer research
Molecular Medicine
chronic lymphocytic leukemia
chromosome aberration
microarray
Fluorescence in situ hybridization
Comparative genomic hybridization
Subjects
Details
- Language :
- English
- ISSN :
- 17558166
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Molecular Cytogenetics
- Accession number :
- edsair.doi.dedup.....75a9492fceec56706c8ad100dc3686d2