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Anti-Inflammatory and Antinociceptive Activities of Bufalin in Rodents
- Source :
- Mediators of Inflammation, Vol 2014 (2014), Mediators of Inflammation
- Publication Year :
- 2014
- Publisher :
- Hindawi Limited, 2014.
-
Abstract
- The aims of this study were to evaluate the anti-inflammatory and analgesic effects of bufalin, a major component of “Chan-su.” We used a carrageenan-induced paw edema model to assess the anti-inflammatory activity of this compound, and Western blot analysis detected NF-κB signaling during this effect. The antinociceptive activities were evaluated by acetic acid-induced writhing, formalin, and hot-plate tests; open-field test investigated effects on the central nervous system. Our data showed that bufalin (0.3 and 0.6 mg/kg, i.p.) potently decreased carrageenan-induced paw edema. Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β(IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α(TNF-α) during these treatments. Further studies demonstrated that bufalin significantly inhibited the activation of NF-κB signaling. Bufalin also reduced acetic acid-induced writhing and the licking time in the formalin test and increased hot-plate reaction latencies. Naloxone pretreatment (2 mg/kg, i.p.) in the early phases of the formalin test and hot-plate test significantly attenuated the bufalin-induced antinociception effects, which suggests the involvement of the opioid system. A reduction in locomotion was not observed in the open-field test after bufalin administration. Taken together, bufalin treatment resulted in in vivo anti-inflammatory and analgesic effects, and bufalin may be a novel, potential drug for the treatment of inflammatory diseases.
- Subjects :
- Male
Article Subject
medicine.drug_class
Interleukin-1beta
Immunology
Analgesic
Anti-Inflammatory Agents
Nitric Oxide Synthase Type II
Pain
(+)-Naloxone
Pharmacology
Carrageenan
Anti-inflammatory
Rats, Sprague-Dawley
Mice
In vivo
lcsh:Pathology
Animals
Edema
Medicine
Inflammation
Analgesics
Mice, Inbred ICR
biology
Interleukin-6
Tumor Necrosis Factor-alpha
business.industry
NF-kappa B
Bufalin
Cell Biology
Rats
Bufanolides
Nitric oxide synthase
Nociception
Cyclooxygenase 2
biology.protein
Licking
business
Research Article
lcsh:RB1-214
Subjects
Details
- ISSN :
- 14661861 and 09629351
- Volume :
- 2014
- Database :
- OpenAIRE
- Journal :
- Mediators of Inflammation
- Accession number :
- edsair.doi.dedup.....75b84161da52719940999c3da59e0393
- Full Text :
- https://doi.org/10.1155/2014/171839