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Accumulation of glycine betaine in transplastomic potato plants expressing choline oxidase confers improved drought tolerance
- Source :
- Planta. 249:1963-1975
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Plastid genome engineering is an effective method to generate drought-resistant potato plants accumulating glycine betaine in plastids. Glycine betaine (GB) plays an important role under abiotic stress, and its accumulation in chloroplasts is more effective on stress tolerance than that in cytosol of transgenic plants. Here, we report that the codA gene from Arthrobacter globiformis, which encoded choline oxidase to catalyze the conversion of choline to GB, was successfully introduced into potato (Solanum tuberosum) plastid genome by plastid genetic engineering. Two independent plastid-transformed lines were isolated and confirmed as homoplasmic via Southern-blot analysis, in which the mRNA level of codA was much higher in leaves than in tubers. GB accumulated in similar levels in both leaves and tubers of codA-transplastomic potato plants (referred to as PC plants). The GB content was moderately increased in PC plants, and compartmentation of GB in plastids conferred considerably higher tolerance to drought stress compared to wild-type (WT) plants. Higher levels of relative water content and chlorophyll content under drought stress were detected in the leaves of PC plants compared to WT plants. Moreover, PC plants presented a significantly higher photosynthetic performance as well as antioxidant enzyme activities during drought stress. These results suggested that biosynthesis of GB by chloroplast engineering was an effective method to increase drought tolerance.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chloroplasts
Drought tolerance
Plant Science
Genetically modified crops
Biology
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Betaine
Bacterial Proteins
Stress, Physiological
Botany
Genetics
Plastids
Arthrobacter
Photosynthesis
Plastid
Solanum tuberosum
Abiotic stress
fungi
food and beverages
Choline oxidase
Plants, Genetically Modified
Droughts
Plant Leaves
Chloroplast
Alcohol Oxidoreductases
030104 developmental biology
chemistry
Genetic Engineering
010606 plant biology & botany
Transplastomic plant
Subjects
Details
- ISSN :
- 14322048 and 00320935
- Volume :
- 249
- Database :
- OpenAIRE
- Journal :
- Planta
- Accession number :
- edsair.doi.dedup.....75c00531f4b8aa72b4f2f3ae47be4478