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ras Oncogene activation in mammary carcinomas induced byn-methyl-n-nitrosourea in copenhagen rats
- Source :
- Molecular Carcinogenesis. 6:260-265
- Publication Year :
- 1992
- Publisher :
- Wiley, 1992.
-
Abstract
- The occurrence of Ha-ras and Ki-ras oncogenes was investigated in mammary tumors produced by treating genetically resistant Copenhagen (Cop) rats with N-methyl-N-nitrosourea. G35-->A codon 12 mutations in both Ha-ras and Ki-ras genes were analyzed by a polymerase chain reaction/liquid hybridization and gel retardation assay. More than half of the adenocarcinomas analyzed contained an activated Ha-ras gene. This was also the predominant mutation in similar tumors from susceptible Buf/N rats, suggesting a common mechanism of initiation. In contrast, only two of 15 mammary adenosquamous carcinomas from the Cop rats contained an activated Ha-ras gene, suggesting a different initiation mechanism for most of these tumors. Ki-ras activation was found in none of five and one of five adenocarcinomas from Buf/N and Cop rats, respectively, and in none of 13 adenosquamous carcinomas from Cop rats. These results suggest that Ki-ras activation does not play a major role in the initiation of the mammary tumors.
- Subjects :
- Cancer Research
medicine.medical_specialty
Adenosquamous carcinoma
Molecular Sequence Data
Mammary gland
Tumor initiation
Adenocarcinoma
Biology
medicine.disease_cause
Polymerase Chain Reaction
Internal medicine
medicine
Animals
Electrophoretic mobility shift assay
Rats, Inbred BUF
Molecular Biology
Gene
Carcinogen
Mutation
Base Sequence
Mammary Neoplasms, Experimental
Methylnitrosourea
Rats, Inbred Strains
DNA, Neoplasm
medicine.disease
Molecular biology
Rats
Gene Expression Regulation, Neoplastic
Genes, ras
Endocrinology
medicine.anatomical_structure
Oligodeoxyribonucleotides
Mutagenesis
Carcinoma, Squamous Cell
Female
Subjects
Details
- ISSN :
- 10982744 and 08991987
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecular Carcinogenesis
- Accession number :
- edsair.doi.dedup.....75c1650ecea0bbbb81ed7674f96bb5f2
- Full Text :
- https://doi.org/10.1002/mc.2940060407