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Laccase-based synthesis of SIC-RED : A new dyeing product for protein gel staining
- Source :
- Biocatalysis and Agricultural Biotechnology 15 (2018), Biocatalysis and Agricultural Biotechnology, 15, 270-276
- Publication Year :
- 2018
-
Abstract
- In the last years, laccases have become leading biocatalysts in green bioprocesses for dye synthesis. Among fungal laccases, the high-redox potential laccase POXA1b from Pleurotus ostreatus is a valuable candidate since it brings together several key-elements: stability and activity in a wide range of pHs and temperatures and the availability of a cost-effective bioprocess for its production. In this work POXA1b catalyzed the coupling of a p-phenylenediamine and α-naphtol, resulting in the production of a new dyeing product, named SIC-RED. The dye was structurally characterized and its performances in protein gel staining tested. The economic feasibility of the process was demonstrated in the case of an Italian small medium enterprise. The results let envisage a real exploitation of the herein enzymatically synthesized dye for protein gel staining.
- Subjects :
- 0301 basic medicine
Proteomics
Bio Process Engineering
Bioengineering
Protein gel staining
01 natural sciences
Applied Microbiology and Biotechnology
Catalysis
03 medical and health sciences
Bioprocess
Laccase
biology
010405 organic chemistry
Chemistry
Economic feasibility
Economic analysis
biology.organism_classification
Combinatorial chemistry
0104 chemical sciences
Staining
High-redox potential laccase
030104 developmental biology
Pleurotus ostreatus
Dyeing
Dye synthesis
Agronomy and Crop Science
Food Science
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 18788181
- Database :
- OpenAIRE
- Journal :
- Biocatalysis and Agricultural Biotechnology 15 (2018), Biocatalysis and Agricultural Biotechnology, 15, 270-276
- Accession number :
- edsair.doi.dedup.....764cd803dcb401711173e54ccf05fe3d