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Biological Insights and NMR Metabolic Profiling of Different Extracts of Spermacoce verticillata (L.) G. Mey
- Source :
- Chemistrybiodiversity. 18(10)
- Publication Year :
- 2021
-
Abstract
- Spermacoce verticillata (L.) G. Mey. is commonly used in the folk medicine by various cultures to manage common diseases. Herein, the chemical and biological profiles of S. verticillata were studied in order to provide a comprehensive characterization of bioactive compounds and also to highlight the therapeutic properties. The in vitro antioxidant activity using free-radical scavenging, phosphomolybdenum, ferrous-ion chelating and reducing power assays, and the inhibitory activity against key enzymes such as acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), tyrosinase, α-amylase and α-glucosidase of S. verticillata extracts (dichloromethane, ethyl acetate, methanol and water) were investigated. The highest total phenolic and flavonoid content were observed in the methanolic and aqueous extracts. Exhaustive 2DNMR investigation has revealed the presence of rutin, ursolic and oleanoic acids. The methanolic extract, followed by aqueous extract have showed remarkable free radical quenching and reducing ability, while the dichloromethane extract was the best source of metal chelators. The tested extracts showed notable inhibitory activity against cholinesterases (AChE: 1.63-4.99 mg GALAE/g extract and BChE: 12.40-15.48 mg GALAE/g extract) and tyrosinase (60.85-159.64 mg KAE/g extract). No inhibitory activity was displayed by ethyl acetate and aqueous extracts against BChE and tyrosinase, respectively. All the tested extracts showed modest α-amylase inhibitory activity, while only the ethyl acetate and aqueous extracts were potent against α-glycosidase. This study further validates the use of S. verticillata in the traditional medicine, while advocating for further investigation for phytomedicine development.
- Subjects :
- Antioxidant
Magnetic Resonance Spectroscopy
Swine
medicine.medical_treatment
Tyrosinase
Flavonoid
Ethyl acetate
antioxidant activity
Bioengineering
NMR, rutin, triterpenoid
Rubiaceae
Saccharomyces cerevisiae
Horse
Biochemistry
Plant Extract
Rutin
chemistry.chemical_compound
Phytomedicine
alpha-Amylase
medicine
Spermacoce verticillata
Enzyme Inhibitor
Animals
Horses
Enzyme Inhibitors
Molecular Biology
Butyrylcholinesterase
chemistry.chemical_classification
Traditional medicine
Animal
Electrophoru
Monophenol Monooxygenase
Plant Extracts
Agaricale
alpha-Glucosidases
General Chemistry
General Medicine
Enzyme
chemistry
Electrophorus
Acetylcholinesterase
Molecular Medicine
alpha-Amylases
biological agent
Agaricales
Subjects
Details
- ISSN :
- 16121880
- Volume :
- 18
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Chemistrybiodiversity
- Accession number :
- edsair.doi.dedup.....76520e7f36cedbcf585f1fa4097d8d1d