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In Vitro Evolution of a Fungal Laccase in High Concentrations of Organic Cosolvents
- Source :
- Chemistry & Biology. 14:1052-1064
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- SummaryFungal laccases are remarkable green catalysts that have a broad substrate specificity and many potential applications in bioremediation, lignocellulose processing, organic synthesis, and more. However, most of these transformations must be carried out at high concentrations of organic cosolvents in which laccases undergo unfolding, thereby losing their activity. We have tailored a thermostable laccase that tolerates high concentrations of cosolvents, the genetic product of five rounds of directed evolution expressed in Saccharomyces cerevisiae. This evolved laccase—R2 variant—was capable of resisting a wide array of cosolvents at concentrations as high as 50% (v/v). Intrinsic laccase features such as the redox potential and the geometry of catalytic coppers varied slightly during the course of the molecular evolution. Some mutations at the protein surface stabilized the laccase by allowing additional electrostatic and hydrogen bonding to occur.
- Subjects :
- CHEMBIOL
Clinical Biochemistry
Saccharomyces cerevisiae
Biochemistry
Redox
Catalysis
Fungal Proteins
chemistry.chemical_compound
Bioremediation
Enzyme Stability
Drug Discovery
Organic chemistry
Organic Chemicals
Molecular Biology
Pharmacology
Laccase
biology
Chemistry
General Medicine
biology.organism_classification
Directed evolution
Mutation
Solvents
Molecular Medicine
Organic synthesis
Directed Molecular Evolution
Systematic evolution of ligands by exponential enrichment
Subjects
Details
- ISSN :
- 10745521
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Chemistry & Biology
- Accession number :
- edsair.doi.dedup.....7658e601f28b98ae299a1842c6fdc80c