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Interacting Genomic Landscapes of REC8-Cohesin, Chromatin, and Meiotic Recombination in Arabidopsis
- Source :
- The Plant Cell
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Meiosis recombines genetic variation and influences eukaryote genome evolution. During meiosis, DNA double-strand breaks (DSBs) enter interhomolog repair to yield crossovers and noncrossovers. DSB repair occurs as replicated sister chromatids are connected to a polymerized axis. Cohesin rings containing the REC8 kleisin subunit bind sister chromatids and anchor chromosomes to the axis. Here, we report the genomic landscape of REC8 using chromatin immunoprecipitation sequencing (ChIP-seq) in Arabidopsis (Arabidopsis thaliana). REC8 associates with regions of high nucleosome occupancy in multiple chromatin states, including histone methylation at H3K4 (expressed genes), H3K27 (silent genes), and H3K9 (silent transposons). REC8 enrichment is associated with suppression of meiotic DSBs and crossovers at the chromosome and fine scales. As REC8 enrichment is greatest in transposon-dense heterochromatin, we repeated ChIP-seq in kyp suvh5 suvh6 H3K9me2 mutants. Surprisingly, REC8 enrichment is maintained in kyp suvh5 suvh6 heterochromatin and no defects in centromeric cohesion were observed. REC8 occupancy within genes anti-correlates with transcription and is reduced in COPIA transposons that reactivate expression in kyp suvh5 suvh6. Abnormal axis structures form in rec8 that recruit DSB-associated protein foci and undergo synapsis, which is followed by chromosome fragmentation. Therefore, REC8 occupancy correlates with multiple chromatin states and is required to organize meiotic chromosome architecture and interhomolog recombination.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Heterochromatin
Chromosomal Proteins, Non-Histone
Arabidopsis
Cell Cycle Proteins
Plant Science
Saccharomyces cerevisiae
Biology
01 natural sciences
Chromosomes, Plant
Chromosomal crossover
03 medical and health sciences
Suppression, Genetic
Meiosis
Histone methylation
Schizosaccharomyces
Sister chromatids
Crossing Over, Genetic
Homologous Recombination
Cohesin
Arabidopsis Proteins
fungi
Synapsis
Cell Biology
DNA Methylation
Chromatin
Cell biology
Nucleosomes
030104 developmental biology
Mutation
DNA Transposable Elements
Genome, Plant
010606 plant biology & botany
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- The Plant Cell
- Accession number :
- edsair.doi.dedup.....766ed53d2911dd4b90ca98319397571e