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Telocytes accompanying cardiomyocyte in primary culture: two- and three-dimensional culture environment
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2010
- Publisher :
- Blackwell Publishing Ltd, 2010.
-
Abstract
- Recently, the presence of telocytes was demonstrated in human and mammalian tissues and organs (digestive and extra-digestive organs, genitourinary organs, heart, placenta, lungs, pleura, striated muscle). Noteworthy, telocytes seem to play a significant role in the normal function and regeneration of myocardium. By cultures of telocytes in two- and three-dimensional environment we aimed to study the typical morphological features as well as functionality of telocytes, which will provide important support to understand their in vivo roles. Neonatal rat cardiomyocytes were isolated and cultured as seeding cells in vitro in two-dimensional environment. Furthermore, engineered myocardium tissue was constructed from isolated cells in three-dimensional collagen/Matrigel scaffolds. The identification of telocytes was performed by using histological and immunohistochemical methods. The results showed that typical telocytes are distributed among cardiomyocytes, connecting them by long telopodes. Telocytes have a typical fusiform cell body with two or three long moniliform telopodes, as main characteristics. The vital methylene blue staining showed the existence of telocytes in primary culture. Immunohistochemistry demonstrated that some c-kit or CD34 immuno-positive cells in engineered heart tissue had the morphology of telocytes, with a typical fusiform cell body and long moniliform telopodes. Also, a significant number of vimentin+ telocytes were present within engineered heart tissue. We suggest that the model of three-dimensional engineered heart tissue could be useful for the ongoing research on the functional relationships of telocytes with cardiomyocytes. Because the heart has the necessary potential of changing the muscle and non-muscle cells during the lifetime, telocytes might play an active role in the heart regeneration process. Moreover, telocytes might be a useful tool for cardiac tissue engineering.
- Subjects :
- Pathology
medicine.medical_specialty
Short Communication
CD34
Cell Culture Techniques
Vimentin
cardiomyocytes
Antigens, CD34
neonatal heart
telocytes
engineered myocardium
symbols.namesake
vimentin
Tissue engineering
three-dimensional culture
Telocyte
medicine
Animals
Humans
Myocytes, Cardiac
Rats, Wistar
Cells, Cultured
Matrigel
biology
telopodes
Tissue Engineering
Regeneration (biology)
Heart
Cell Biology
Interstitial Cells of Cajal
Interstitial cell of Cajal
Rats
Proto-Oncogene Proteins c-kit
Animals, Newborn
Cell culture
c-kit
symbols
biology.protein
Molecular Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 15824934 and 15821838
- Volume :
- 14
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....7698716d20bdee46f93b5fd2db388611