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Comparative Transcriptome Analysis between Cytoplasmic Male-sterile Line and Its Maintainer During the Floral Bud Development of Pepper
- Source :
- Horticultural Plant Journal, Vol 6, Iss 2, Pp 89-98 (2020)
- Publication Year :
- 2020
- Publisher :
- KeAi Communications Co., Ltd., 2020.
-
Abstract
- The male-sterile line has been largely used in the hybrid seed production of pepper, which can effectively improve the efficiency of hybrid seed production. However, the formation mechanism of male sterility in pepper remains unclear. In the present study, we compared the gene expression patterns between pepper cytoplasmic male sterile line 9704A and its maintainer 9704B during floral bud development using RNA sequencing technology. A total of 547 976 and 2 416 Differentially Expressed Genes (DEGs) were identified in the stage S1, S2 and S3, respectively, and more than 70% of the DEGs were down-regulated in the sterile line. Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to further understand the functions of these identified DEGs. The results showed that the DEGs were mainly enriched in pathways of starch and sucrose metabolism, pentose and glucuronate interconversions. A number of genes, such as MS1, PME5, ATPB, and lots of transcription factors were found down-regulated in the sterile line, and we also identified a series of genes with large differences in expression patterns between sterile line and maintainer line. Collectively, our findings laid a foundation for further molecular breeding in pepper and provided new insights into its mechanism underlying the male sterility. Keywords: pepper, capsicum annuum, cytoplasmic male sterility, comparative transcriptome analysis, DEGs
- Subjects :
- 0106 biological sciences
0301 basic medicine
Genetics
Ecology
Renewable Energy, Sustainability and the Environment
Sterility
Glucuronate
Software maintainer
fungi
food and beverages
Plant Science
Biology
lcsh:Plant culture
01 natural sciences
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Transcriptome
03 medical and health sciences
030104 developmental biology
Gene expression
Pepper
lcsh:SB1-1110
KEGG
Gene
Ecology, Evolution, Behavior and Systematics
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 24680141
- Volume :
- 6
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Horticultural Plant Journal
- Accession number :
- edsair.doi.dedup.....76dbd02e798df96885ece36a3f762921