Additional file 1: of N6-methyldeoxyadenosine directs nucleosome positioning in Tetrahymena DNA

Figure S1. Preferred sequence motifs for Tetrahymena 6mA sites. Figure S2. Agarose gel of MNase digested nuclei. Figure S3. Nucleosome assembly favors 6mA-free regions in vitro. Figure S4. Changes of mean value after 6mA modification in the intra-base pair (A-F) and inter-base pair (G-L) parameters as a function of distance from the center of the modification site. Figure S5. Changes of standard deviation (s.d.) after 6mA modification in the intra-base pair (A-F) and inter-base pair (G-L) parameters as a function of distance from the center of AT. Figure S6. Multiple sequence alignment and phylogenetic distribution analysis of MTA70 family. Figure S7. Knockout of two methyltransferases in Tarahymena and in vitro methylation activity characterization of methytransferaseTAMT-1. Figure S8. Effects of tamt-1 knockout. (DOC 11096 kb)