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Assessment of mismatch repair deficiency in ovarian cancer
- Source :
- Journal of Medical Genetics
- Publication Year :
- 2020
-
Abstract
- BackgroundHereditary causes of ovarian cancer include Lynch syndrome, which is due to inherited pathogenic variants affecting one of the four mismatch repair genes involved in DNA repair. The aim of this study was to evaluate tumour mismatch repair deficiency and prevalence of Lynch syndrome in high-risk women referred to the Manchester Centre for Genomic Medicine with ovarian cancer over the past 20 years.MethodsWomen with ovarian cancer diagnosed before the age of 35 years and/or with a suggestive personal or family history of Lynch syndrome cancers underwent tumour testing with immunohistochemistry for mismatch repair deficiency and, where indicated, MLH1 promoter methylation testing followed by constitutional testing for Lynch syndrome.ResultsIn total, 261 ovarian cancers were tested and 27 (10.3%; 95% CI 6.9% to 14.7%) showed mismatch repair deficiency by immunohistochemistry. Three of 7 with MLH1 loss showed MLH1 promoter hypermethylation, and 18 of the remaining 24 underwent constitutional testing for Lynch syndrome. A further 15 women with mismatch repair proficient tumours underwent constitutional testing because of a strong family history of Lynch syndrome cancers. Pathogenic variants were identified in 9/33 (27%) women who underwent constitutional testing, aged 33–59 years (median 48 years), including one whose tumour was mismatch repair proficient. Most Lynch syndrome tumours were of endometrioid histological subtype.ConclusionsTumour mismatch repair deficiency identified by immunohistochemistry is a useful prescreen for constitutional testing in women with ovarian cancer with personal or family histories suggestive of Lynch syndrome.
- Subjects :
- 0301 basic medicine
Oncology
DNA Mismatch Repair
0302 clinical medicine
Surgical oncology
Medicine
Family history
Genetics (clinical)
Sequence Deletion
Ovarian Neoplasms
medicine.diagnostic_test
Immunohistochemistry
Lynch syndrome
030220 oncology & carcinogenesis
DNA mismatch repair
Female
Microsatellite Instability
MutL Protein Homolog 1
Adult
medicine.medical_specialty
congenital, hereditary, and neonatal diseases and abnormalities
DNA repair
MLH1
genetic testing
03 medical and health sciences
Young Adult
surgical oncology
Internal medicine
Genetics
Cancer Genetics
Humans
Genetic Predisposition to Disease
Alleles
Genetic Association Studies
Germ-Line Mutation
Genetic testing
Neoplasm Staging
business.industry
gynecology
nutritional and metabolic diseases
DNA Methylation
medicine.disease
digestive system diseases
030104 developmental biology
Mutation
Neoplasm Grading
business
Ovarian cancer
DNA Damage
Subjects
Details
- ISSN :
- 14686244
- Volume :
- 58
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of medical genetics
- Accession number :
- edsair.doi.dedup.....7731123823f44254a0b76b16d2360a7b