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Fluorescence biosensor for real-time interaction dynamics of host proteins with HIV-1 capsid tubes
- Publication Year :
- 2019
- Publisher :
- Cold Spring Harbor Laboratory, 2019.
-
Abstract
- The human immunodeficiency virus 1 (HIV-1) capsid serves as a binding platform for proteins and small molecules from the host cell that regulate various steps in the virus life cycle. However, there are currently no quantitative methods that use assembled capsid lattices for measuring host-pathogen interaction dynamics. Here we developed a single molecule fluorescence biosensor using self-assembled capsid tubes as biorecognition elements and imaged capsid binders using total internal reflection fluorescence microscopy in a microfluidic setup. The method is highly sensitive in its ability to observe and quantify binding, obtain dissociation constants, extract kinetics with an extended application of using more complex analytes that can accelerate characterisation of novel capsid binders.
- Subjects :
- Materials science
viruses
Human immunodeficiency virus (HIV)
Biosensing Techniques
medicine.disease_cause
03 medical and health sciences
Capsid
0302 clinical medicine
Viral life cycle
Lab-On-A-Chip Devices
Microscopy
medicine
Humans
General Materials Science
Interaction dynamics
030223 otorhinolaryngology
Host protein
030304 developmental biology
0303 health sciences
Total internal reflection fluorescence microscope
Chemistry
Fluorescence biosensor
Single-molecule experiment
Fluorescence
Small molecule
3. Good health
Microscopy, Fluorescence
HIV-1
Biophysics
Biosensor
030217 neurology & neurosurgery
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....773c8ad405e2808a3e91889bbd4e22e0