Monozygotic multiple gestation after intracytoplasmic sperm injection and preimplantation genetic diagnosis

Objective To report a possible association between intracytoplasmic sperm injection (ICSI)–preimplantation genetic diagnosis (PGD) and monozygotic multiple gestation. Design Small case series. Setting In vitro fertilization unit in an academic medical center. Patient(s) Three patients were treated with ICSI-PGD for sexing as well as selection against a known translocation. Intervention(s) Transfer of day 4 embryos to the uterus. Main Outcome Measure(s) Clinical pregnancy. Result(s) Two pairs of monozygotic twins and a triplet pregnancy. Conclusion(s) Repeated manipulation of the zona pellucida as well as extended embryo culture during ICSI-PGD treatments may result in monozygotic twin and triplet pregnancies.