Back to Search
Start Over
Acclimation to salt modifies the activation of several osmotic stress-activated lipid signalling pathways in Chlamydomonas
- Source :
- Phytochemistry, 135, 64-72. Elsevier
- Publication Year :
- 2016
-
Abstract
- Osmotic stress rapidly activates several phospholipid signalling pathways in the unicellular alga Chlamydomonas. In this report, we have studied the effects of salt-acclimation on growth and phospholipid signalling. Growing cells on media containing 100 mM NaCl increased their salt-tolerance but did not affect the overall phospholipid content, except that levels of phosphatidylinositol phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] were reduced by one-third. When these NaCl-acclimated cells were treated with increasing concentrations of salt, the same lipid signalling pathways as in non-acclimated cells were activated. This was witnessed as increases in phosphatidic acid (PA), lyso-phosphatidic acid (L-PA), diacylglycerol pyrophosphate (DGPP), PI(4,5)P2 and its isomer PI(3,5)P2. However, all dose-dependent responses were shifted to higher osmotic-stress levels, and the responses were lower than in non-acclimated cells. When NaCl-acclimated cells were treated with other osmotica, such as KCl and sucrose, the same effects were found, illustrating that they were due to hyperosmotic rather than hyperionic acclimation. The results indicate that acclimation to moderate salt stress modifies stress perception and the activation of several downstream pathways.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Glycerol
Sucrose
Osmotic shock
Acclimatization
Phospholipid
Phosphatidic Acids
Plant Science
Horticulture
Biology
Sodium Chloride
01 natural sciences
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Isomerism
Osmotic Pressure
Phospholipase D
Phosphatidylinositol
Molecular Biology
Osmotic concentration
Chlamydomonas
General Medicine
Phosphatidic acid
Salt Tolerance
Plants
Phosphate
biology.organism_classification
Cell biology
Diphosphates
030104 developmental biology
chemistry
Signal transduction
010606 plant biology & botany
Signal Transduction
Subjects
Details
- ISSN :
- 18733700 and 00319422
- Volume :
- 135
- Database :
- OpenAIRE
- Journal :
- Phytochemistry
- Accession number :
- edsair.doi.dedup.....7772665a2da3ec56402e031dbf920412