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Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression
- Source :
- International journal of biological macromolecules. 183
- Publication Year :
- 2021
-
Abstract
- Radix Astragali polysaccharide RAP has been reported to play a crucial role in hematopoiesis without a clear mechanism. In this study, RAP's effects to enhance the recovery of cyclophosphamide (Cy)-suppressed bone marrow and blood cells is confirmed in vivo first. Confocal micrographs demonstrated the interesting direct binding of FITC-RAP to hematopoietic stem cells (HSC) in bone marrow. RAP protects both mice and human HSC in terms of cell morphology, proliferation, and apoptosis. RNA-sequencing and shRNA approaches revealed FOS to be a key regulator in RAP's protection. These evidences provide an unreported mechanism that RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression.
- Subjects :
- Cell Survival
02 engineering and technology
Cell morphology
Biochemistry
Small hairpin RNA
03 medical and health sciences
Mice
Structural Biology
In vivo
Polysaccharides
medicine
Animals
Humans
Molecular Biology
Cyclophosphamide
030304 developmental biology
Cell Proliferation
0303 health sciences
Chemistry
Sequence Analysis, RNA
fungi
Hematopoietic stem cell
General Medicine
Astragalus propinquus
021001 nanoscience & nanotechnology
Hematopoietic Stem Cells
Hematopoiesis
Up-Regulation
body regions
Haematopoiesis
medicine.anatomical_structure
Apoptosis
Cancer research
Female
sense organs
Bone marrow
Stem cell
0210 nano-technology
Proto-Oncogene Proteins c-fos
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 183
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....77884824715cfd710f85e933a66169d9