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A small-angle X-ray scattering study of alpha-synuclein from human red blood cells
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- Katsuya Araki, Naoto Yagi, Rie Nakatani, Hiroshi Sekiguchi, Masatomo So, Hisashi Yagi, Noboru Ohta, Yoshitaka Nagai, Yuji Goto & Hideki Mochizuki "A small-angle X-ray scattering study of alpha-synuclein from human red blood cells", Scientific Reports, volume 6, Article number: 30473, Springer Nature, 2016<br />α-synuclein (α-syn) is the main component of Lewy bodies, which are neuropathological hallmarks of patients with Parkinson’s disease. As it has been controversial whether human α-syn from erythrocytes exists as a tetramer under physiological conditions, we tried solving this issue by the small-angle X-ray solution scattering method. Under two different conditions (high ionic strength with a Tris buffer and low ionic strength with an ammonium acetate buffer), no evidence was found for the presence of tetramer. When comparing erythrocyte and recombinant α-syn molecules, we found no significant difference of the molecular weight and the secondary structure although the buffer conditions strongly affect the radius of gyration of the protein. The results indicate that, even though a stable tetramer may not be formed, conformation of α-syn depends much on its environment, which may be the reason for its tendency to aggregate in cells.
- Subjects :
- 0301 basic medicine
Tris
Erythrocytes
Biology
Article
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
X-Ray Diffraction
Tetramer
Scattering, Small Angle
Humans
Protein secondary structure
Alpha-synuclein
Multidisciplinary
Small-angle X-ray scattering
Recombinant Proteins
030104 developmental biology
chemistry
Biochemistry
Ionic strength
Chromatography, Gel
alpha-Synuclein
Radius of gyration
Biophysics
Ammonium acetate
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....77907d85a203889c1c5d3b03044fcefb
- Full Text :
- https://doi.org/10.1038/srep30473