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Specific Urinary Metabolites in Malignant Melanoma
- Source :
- Medicina, Volume 55, Issue 5, Medicina; Volume 55; Issue 5; Pages: 145, Medicina, Vol 55, Iss 5, p 145 (2019)
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- Background and objectives: Melanin, which has a confirmed role in melanoma cell behaviour, is formed in the process of melanogenesis and is synthesized from tryptophan, L-tyrosine and their metabolites. All these metabolites are easily detectable by chromatography in urine. Materials and Methods: Urine samples of 133 individuals (82 malignant melanoma patients and 51 healthy controls) were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC). The diagnosis of malignant melanoma was confirmed histologically. Results: Chromatograms of melanoma patients showed increased levels of 5,6-dihydroxyindole-2-carboxylic acid, vanilmandelic acid, homovanilic acid, tryptophan, 5-hydroxyindole-3-acetic acid, and indoxyl sulphate compared to healthy controls. Concentration of indoxyl sulphate, homovanilic acid and tryptophan were significantly increased even in the low clinical stage 0 of the disease (indoxyl sulphate, homovanilic acid and tryptophan in patients with clinical stage 0 vs. controls expressed as medium/ interquartile range in &micro<br />mol/mmol creatinine: 28.37/15.30 vs. 5.00/6.91<br />47.97/33.08 vs. 7.33/21.25<br />and 16.38/15.98 vs. 3.46/6.22, respectively). Conclusions: HPLC detection of metabolites of L-tyrosine and tryptophan in the urine of melanoma patients may play a significant role in diagnostics as well as a therapeutic strategy of melanoma cancer.
- Subjects :
- Adult
Male
Medicine (General)
HPLC
urine
malignant melanoma
metabolite
5,6-dihydroxyindole-2-carboxylic acid
vanilmandelic acid
homovanilic acid
tryptophan
5-hydroxyindole-3-acetic acid
indoxyl sulphate
Indoles
Metabolite
Urine
Pharmacology
High-performance liquid chromatography
Article
Melanin
chemistry.chemical_compound
R5-920
Biomarkers, Tumor
medicine
Humans
Vanilmandelic Acid
Melanoma
Aged
Creatinine
business.industry
Tryptophan
Homovanillic Acid
General Medicine
Hydroxyindoleacetic Acid
Middle Aged
medicine.disease
chemistry
Female
business
Indican
Subjects
Details
- Language :
- English
- ISSN :
- 1010660X
- Database :
- OpenAIRE
- Journal :
- Medicina
- Accession number :
- edsair.doi.dedup.....77b8da865e2a62c40a2f1973dac23e95
- Full Text :
- https://doi.org/10.3390/medicina55050145