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The effect of elevated temperature on the aggregation of African green monkey kidney cells

Authors :
Margaret Tzaphlidou
Demetrios P. Matthopoulos
Source :
Cell Biology International Reports. 12:413-424
Publication Year :
1988
Publisher :
Wiley, 1988.

Abstract

The aggregation kinetics of African green monkey kidney cells CV1 and of the SV40 transformed derivative COS1 cells that had been incubated at 37 degrees C or 43.5 degrees C was studied using the shaking flask system. COS1 cells show a three fold decrease in aggregation rate compared to CV1 cells when both cell types were incubated and aggregated at 37 degrees C. When these cell types were incubated at 43.5 degrees C for 5 hours, then aggregated at 37 degrees C showed a faster aggregation kinetics than before. Their aggregation at 43.5 degrees C with prior incubation at 37 degrees C or 43.5 degrees C reached the aggregation kinetics of 43.5 degrees C incubated cells aggregated at 37 degrees C. The addition of serum in the aggregation medium did not influence extensively the aggregation rates of both cell types.

Details

ISSN :
03091651
Volume :
12
Database :
OpenAIRE
Journal :
Cell Biology International Reports
Accession number :
edsair.doi.dedup.....77c04feb524bc47b922f75bb19b08938