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Quantification of glial fibrillary acidic protein: comparison of slot-immunobinding assays with a novel sandwich ELISA
Quantification of glial fibrillary acidic protein: comparison of slot-immunobinding assays with a novel sandwich ELISA
- Source :
- Neurotoxicology and teratology. 13(3)
- Publication Year :
- 1991
-
Abstract
- Detailed protocols are presented for assaying glial fibrillary acidic protein (GFAP), an astrocyte localized protein which serves as a quantitative marker of toxicant-induced injury to the central nervous system. Two different solid-phase assay procedures are described: 1) a nitrocellulose based slot-immunobinding assay and 2) a novel microtiter plate based sandwich ELISA. The performance of both assays was assessed by measuring the content of GFAP in homogenates of specific regions of the rat brain and in homogenates of brain regions damaged by the prototype neurotoxicants, trimethyltin (TMT) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Both procedures gave similar results that were consistent with previously published observations. By comparing the simplicity, cost effectiveness, safety and speed of the two methods, it appears likely that the sandwich ELISA has several advantages over slot-immunobinding assays.
- Subjects :
- Cost effectiveness
Immunoblotting
Pyramidal Tracts
Enzyme-Linked Immunosorbent Assay
Toxicology
Hippocampus
Antibodies
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Microtiter plate
Mice
Developmental Neuroscience
Glial Fibrillary Acidic Protein
medicine
Animals
chemistry.chemical_classification
Brain Chemistry
Neurons
Glial fibrillary acidic protein
biology
MPTP
Antibodies, Monoclonal
Brain
MPTP Poisoning
Molecular biology
Rats
Mice, Inbred C57BL
medicine.anatomical_structure
chemistry
Spinal Cord
biology.protein
Female
Glycoprotein
Nitrocellulose
Quantitative analysis (chemistry)
Biomarkers
Astrocyte
Subjects
Details
- ISSN :
- 08920362
- Volume :
- 13
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Neurotoxicology and teratology
- Accession number :
- edsair.doi.dedup.....77e8d2ace54a1fb7b9770628147d8c97