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Elp1 facilitates RAD51-mediated homologous recombination repair via translational regulation
- Source :
- Journal of Biomedical Science, Journal of Biomedical Science, Vol 28, Iss 1, Pp 1-19 (2021)
- Publication Year :
- 2021
- Publisher :
- BioMed Central, 2021.
-
Abstract
- Background RAD51-dependent homologous recombination (HR) is one of the most important pathways for repairing DNA double-strand breaks (DSBs), and its regulation is crucial to maintain genome integrity. Elp1 gene encodes IKAP/ELP1, a core subunit of the Elongator complex, which has been implicated in translational regulation. However, how ELP1 contributes to genome maintenance is unclear. Methods To investigate the function of Elp1, Elp1-deficient mouse embryonic fibroblasts (MEFs) were generated. Metaphase chromosome spreading, immunofluorescence, and comet assays were used to access chromosome abnormalities and DSB formation. Functional roles of Elp1 in MEFs were evaluated by cell viability, colony forming capacity, and apoptosis assays. HR-dependent DNA repair was assessed by reporter assay, immunofluorescence, and western blot. Polysome profiling was used to evaluate translational efficiency. Differentially expressed proteins and signaling pathways were identified using a label-free liquid chromatography–tandem mass spectrometry (LC–MS/MS) proteomics approach. Results Here, we report that Elp1 depletion enhanced genomic instability, manifested as chromosome breakage and genotoxic stress-induced genomic DNA fragmentation upon ionizing radiation (IR) exposure. Elp1-deficient cells were hypersensitive to DNA damage and exhibited impaired cell proliferation and defective HR repair. Moreover, Elp1 depletion reduced the formation of IR-induced RAD51 foci and decreased RAD51 protein levels. Polysome profiling analysis revealed that ELP1 regulated RAD51 expression by promoting its translation in response to DNA damage. Notably, the requirement for ELP1 in DSB repair could be partially rescued in Elp1-deficient cells by reintroducing RAD51, suggesting that Elp1-mediated HR-directed repair of DSBs is RAD51-dependent. Finally, using proteome analyses, we identified several proteins involved in cancer pathways and DNA damage responses as being differentially expressed upon Elp1 depletion. Conclusions Our study uncovered a molecular mechanism underlying Elp1-mediated regulation of HR activity and provides a novel link between translational regulation and genome stability.
- Subjects :
- Genome instability
Translational efficiency
DNA damage
DNA repair
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
RAD51
Biology
Genomic Instability
Mice
Translational regulation
Animals
Pharmacology (medical)
Homologous recombination
Molecular Biology
Elp1
Research
Biochemistry (medical)
Intracellular Signaling Peptides and Proteins
Recombinational DNA Repair
Chromosome Breakage
Cell Biology
General Medicine
Fibroblasts
Cell biology
Protein Biosynthesis
Medicine
Rad51 Recombinase
Chromosome breakage
Subjects
Details
- Language :
- English
- ISSN :
- 14230127 and 10217770
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Science
- Accession number :
- edsair.doi.dedup.....77ec89236beaeb1742eca2d74b6e65b7