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Sensitivity to chilling of medaka (Oryzias latipes) embryos at various developmental stages
- Source :
- Theriogenology. 64:112-122
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- As an essential step toward cryopreservation of fish embryos, we examined the chilling sensitivity of medaka (Oryzias latipes) embryos at various developmental stages. Embryos at the 2-4 cell, 8-16 cell, morula, blastula, and early gastrula stages were suspended in Hanks solution. They were chilled to various temperatures (usually 0 degrees C), kept for various periods (usually 20 min), then cultured for up to 14 d to determine survival (assessed by the ability to hatch). Embryos at the 2-4 cell stage were the most sensitive to chilling to 0 degrees C, but sensitivity decreased as development proceeded. The survival rate of 2-4 cell embryos was affected after 2 min of chilling at 0 degrees C; although the rate decreased gradually as the duration of chilling increased, 38% of them still survived after 40 min of chilling. Embryos at the 2-4 cell stage were sensitive to chilling at 0 or -5 degrees C, but much less sensitive at 5 or 10 degrees C. The survival rate of 2-4 cell embryos subjected to repeated rapid cooling and warming was similar to that of those kept chilled. When early gastrula embryos were preserved at 0 or 5 degrees C, the hatching rate did not decrease after 12 and 24h of chilling, respectively, but then decreased gradually as storage was prolonged; however, 3-10% of the embryos hatched even after storage for 10 d. In conclusion, although later-stage medaka embryos would be suitable for cryopreservation (from the perspective of chilling sensitivity), chilling injury may not be serious in earlier stage embryos.
- Subjects :
- Time Factors
animal structures
Oryzias
Embryonic Development
Cryopreservation
Embryo Culture Techniques
Andrology
Food Animals
parasitic diseases
Botany
Animals
Chilling injury
Small Animals
biology
Equine
Hatching
fungi
Embryogenesis
food and beverages
Embryo
Gastrula
biology.organism_classification
Blastula
Cold Temperature
embryonic structures
Fish
Animal Science and Zoology
Tissue Preservation
Subjects
Details
- ISSN :
- 0093691X
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Theriogenology
- Accession number :
- edsair.doi.dedup.....77ef66accb13737417b9f75983d125d3
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2004.11.006