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Protocatechuic acid suppresses ovalbumin-induced airway inflammation in a mouse allergic asthma model
- Source :
- International Immunopharmacology. 15:780-788
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Protocatechuic acid (PCA) has been isolated from the leaves of ilex chinenses and has numerous pharmacologic effects, including anti-inflammatory and antitumoral activities. This study aims to evaluate the antiasthma activity of PCA and investigate its possible molecular mechanisms. BALB/c mice were sensitized and challenged to ovalbumin (OVA).Then mice were intraperitoneally (i.p.) injected with PCA 1h before OVA challenge. We found that PCA treatment at 15 or 30 mg/kg significantly decreased OVA-induced airway hyper-responsiveness (AHR) to inhaled methacholine. Type 2 helper T cell (Th2) cytokines in bronchoalveolar lavage (BAL) fluid, such as interleukin-4 (IL-4), interleukin 5 (IL-5) and interleukin-13 (IL-13), and serum OVA-specific immunoglobulin E (IgE) levels, were also reduced by PCA. Moreover treatment with PCA markedly decreased the number of inflammatory cells in BALF and attenuated OVA-induced mRNA expression of CCl11, CCR3, Muc5ac, acidic mammalian chitinase (AMCase), chitinase 3-like protein 4 (Ym2) and E-selectin in lung tissues, lung histopathological studies showed that PCA inhibited inflammatory cell infiltration and mucus hypersecretion compared with the OVA-induced mice group. We then investigated the possible molecular mechanisms which might be implicated in PCA activity. Our results suggested that the protective effect of PCA might be mediated by the inhibition of the extracellular signal-regulated protein kinase (ERK), p38 Mitogen-activated protein kinase (MAPK) phosphorylation and the nuclear factor-κB (NF-κB) activation.
- Subjects :
- MAPK/ERK pathway
Ovalbumin
p38 mitogen-activated protein kinases
Blotting, Western
Immunology
CCR3
Pharmacology
Immunoglobulin E
Mice
Administration, Inhalation
Hydroxybenzoates
Respiratory Hypersensitivity
medicine
Animals
Immunology and Allergy
Anti-Asthmatic Agents
Protein kinase A
Lung
Interleukin 5
Methacholine Chloride
Mice, Inbred BALB C
Dose-Response Relationship, Drug
medicine.diagnostic_test
biology
respiratory system
Asthma
Bronchoalveolar lavage
biology.protein
Cytokines
Female
Bronchoalveolar Lavage Fluid
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 15675769
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- International Immunopharmacology
- Accession number :
- edsair.doi.dedup.....782bfe0c9c777161ec96cb1e9fbe971e