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Differential Recognition of Terminal Extracellular Plasmodium falciparum VAR2CSA Domains by Sera from Multigravid, Malaria-Exposed Malian Women

Authors :
Andrea A. Berry
Jozelyn Pablo
J. Alexandra Rowe
Miriam K. Laufer
Kirsten E. Lyke
Jason A. Bailey
Matthew Adams
Amadou Niangaly
Algis Jasinskas
Mark A. Travassos
Matthew B. Laurens
Amed Ouattara
Philip L. Felgner
Bourema Kouriba
Mahamadou A. Thera
Ogobara K. Doumbo
Shannon Takala-Harrison
Rie Nakajima
Christopher V. Plowe
Abdoulaye K. Kone
Myaing M. Nyunt
Drissa Coulibaly
Source :
The American Journal of Tropical Medicine and Hygiene, Travassos, M A, Coulibaly, D, Bailey, J A, Niangaly, A, Adams, M, Nyunt, M M, Ouattara, A, Lyke, K E, Laurens, M B, Pablo, J, Jasinskas, A, Nakajima, R, Berry, A A, Takala-Harrison, S, Kone, A K, Kouriba, B, Rowe, J A, Doumbo, O K, Thera, M A, Laufer, M K, Felgner, P L & Plowe, C V 2015, ' Differential recognition of terminal extracellular Plasmodium falciparum VAR2CSA domains by sera from multigravid, malaria-exposed Malian women ', American Journal of Tropical Medicine and Hygiene, vol. 92, no. 6, pp. 1190-1194 . https://doi.org/10.4269/ajtmh.14-0524
Publication Year :
2015
Publisher :
The American Society of Tropical Medicine and Hygiene, 2015.

Abstract

The Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family mediates parasite sequestration in small capillaries through tissue-specific cytoadherence. The best characterized of these proteins is VAR2CSA, which is expressed on the surface of infected erythrocytes that bind to chondroitin sulfate in the placental matrix. Antibodies to VAR2CSA prevent placental cytoadherence and protect against placental malaria. The size and complexity of the VAR2CSA protein pose challenges for vaccine development, but smaller constitutive domains may be suitable for subunit vaccine development. A protein microarray was printed to include five overlapping fragments of the 3D7 VAR2CSA extracellular region. Malian women with a history of at least one pregnancy had antibody recognition of four of these fragments and had stronger reactivity against the two distal fragments than did nulliparous women, children, and men from Mali, suggesting that the C-terminal extracellular VAR2CSA domains are a potential focus of protective immunity. With carefully chosen sera from longitudinal studies of pregnant women, this approach has the potential to identify seroreactive VAR2CSA domains associated with protective immunity against pregnancy-associated malaria.

Details

Language :
English
ISSN :
14761645 and 00029637
Volume :
92
Issue :
6
Database :
OpenAIRE
Journal :
The American Journal of Tropical Medicine and Hygiene
Accession number :
edsair.doi.dedup.....78524eb8d91aeffa9451ec84bd4e165b
Full Text :
https://doi.org/10.4269/ajtmh.14-0524