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An in vitro evaluation of standard rotational thromboelastography in monitoring of effects of recombinant factor VIIa on coagulopathy induced by hydroxy ethyl starch
- Source :
- BMC Blood Disorders, Vol 5, Iss 1, p 3 (2005), BMC Blood Disorders
- Publication Year :
- 2005
- Publisher :
- Springer Science and Business Media LLC, 2005.
-
Abstract
- Background Rotational thromboelastography (ROTEG) has been proposed as a monitoring tool that can be used to monitor treatment of hemophilia with recombinant factor VIIa (rFVIIa). In these studies special non-standard reagents were used as activators of the coagulation. The aim of this study was to evaluate if standard ROTEG analysis could be used for monitoring of effects of recombinant factor VIIa (rFVIIa) on Hydroxy Ethyl Starch-induced dilutional coagulopathy. Methods The study was performed in vitro on healthy volunteers. Prothrombin time (PT) and ROTEG analysis were performed after dilution with 33% hydroxy ethyl starch and also after addition of rFVIIa to the diluted blood. Results PT was impaired with INR changing from 0.9 before dilution to 1.2 after dilution while addition of rFVIIa to diluted blood lead to an overcorrection of the PT to an International Normalized Ratio (INR) value of 0.6 (p = 0.01). ROTEG activated with the contact activator ellagic acid was impaired by hemodilution (p = 0.01) while addition of rFVIIa had no further effects. ROTEG activated with tissue factor (TF) was also impaired by hemodilution (p = 0.01) while addition of rFVIIa lead to further impairment of the coagulation (p = 0.01). Conclusions The parameters affected in the ROTEG analysis were Clot Formation Time and Amplitude after 15 minutes while the Clotting Time was unaffected. We believe these effects to be due to methodological problems when using standard activators of the coagulation in the ROTEG analysis in combination with rFVIIa.
- Subjects :
- Prothrombin time
medicine.medical_specialty
Hematology
biology
medicine.diagnostic_test
lcsh:RC633-647.5
business.industry
lcsh:Diseases of the blood and blood-forming organs
Pharmacology
medicine.disease
Surgery
Thromboelastometry
Tissue factor
Coagulation
Clotting time
Recombinant factor VIIa
Internal medicine
medicine
biology.protein
Coagulopathy
business
Molecular Biology
Research Article
Subjects
Details
- ISSN :
- 20521839
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- BMC Hematology
- Accession number :
- edsair.doi.dedup.....7855a5994a20fdc3bf436fca75776c5e
- Full Text :
- https://doi.org/10.1186/1471-2326-5-3