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Evaluation of a bioaerosol sampler for indoor environmental surveillance of Severe Acute Respiratory Syndrome Coronavirus 2
- Source :
- PLoS ONE, Vol 16, Iss 11, p e0257689 (2021), PLoS ONE, Vol 16, Iss 11 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- The worldwide spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has ubiquitously impacted many aspects of life. As vaccines continue to be manufactured and administered, limiting the spread of SARS-CoV-2 will rely more heavily on the early identification of contagious individuals occupying reopened and increasingly populated indoor environments. In this study, we investigated the utility of an impaction-based bioaerosol sampling system with multiple nucleic acid collection media. Heat-inactivated SARS-CoV-2 was utilized to perform bench-scale, short-range aerosol, and room-scale aerosol experiments. Through bench-scale experiments, AerosolSense Capture Media (ACM) and nylon flocked swabs were identified as the highest utility media. In room-scale aerosol experiments, consistent detection of aerosol SARS-CoV-2 was achieved at an estimated aerosol concentration equal to or greater than 0.089 genome copies per liter of room air (gc/L) when air was sampled for eight hours or more at less than one air change per hour (ACH). Shorter sampling periods (75 minutes) yielded consistent detection at ~31.8 gc/L of room air and intermittent detection down to ~0.318 gc/L at (at both 1 and 6 ACH). These results support further exploration in real-world testing scenarios and suggest the utility of indoor aerosol surveillance as an effective risk mitigation strategy in occupied buildings.
- Subjects :
- RNA viruses
Viral Diseases
Atmospheric Science
Veterinary medicine
Coronaviruses
Medical Conditions
Built Environment
Materials
Pathology and laboratory medicine
Virus Testing
Multidisciplinary
Dust
Genomics
Limiting
Medical microbiology
Terrestrial Environments
Infectious Diseases
Physical Sciences
Viruses
RNA, Viral
Medicine
SARS CoV 2
Pathogens
Environmental Monitoring
Research Article
Bioaerosol
SARS coronavirus
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Science
Materials Science
Genome, Viral
Microbiology
Meteorology
Diagnostic Medicine
Air change
Genetics
Humans
Aerosols
Medicine and health sciences
Biology and life sciences
SARS-CoV-2
Environmental surveillance
allergology
Ecology and Environmental Sciences
Organisms
Viral pathogens
COVID-19
Covid 19
Humidity
Microbial pathogens
Aerosol
Sampling system
Mixtures
Earth Sciences
Room air distribution
Environmental science
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....785783da75ca8ec7a5275811f9259109