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Mammalian cell growth versus biofilm formation on biomaterial surfaces in an in vitro post-operative contamination model
- Source :
- Microbiology-Sgm, 156, 3073-3078. MAIK NAUKA/INTERPERIODICA/SPRINGER
- Publication Year :
- 2010
-
Abstract
- Biomaterial-associated infections are the major cause of implant failure and can develop many years after implantation. Success or failure of an implant depends on the balance between host tissue integration and bacterial colonization. Here, we describe a newin vitromodel for the post-operative bacterial contamination of implant surfaces and investigate the effects of contamination on the balance between mammalian cell growth and bacterial biofilm formation. U2OS osteosarcoma cells were seeded on poly(methyl methacrylate) in different densities and allowed to grow for 24 h in a parallel-plate flow chamber at a low shear rate (0.14 s−1), followed by contamination withStaphylococcus epidermidisATCC 35983 at a shear rate of 11 s−1. The U2OS cells and staphylococci were allowed to grow simultaneously for another 24 h under low-shear conditions (0.14 s−1). Mammalian cell growth was severely impaired when the bacteria were introduced to surfaces with a low initial cell density (2.5×104cells cm−2), but in the presence of higher initial cell densities (8.2×104cells cm−2and 17×104cells cm−2), contaminating staphylococci did not affect cell growth. This study is believed to be the first to show that a critical coverage by mammalian cells is needed to effectively protect a biomaterial implant against contaminating bacteria.
- Subjects :
- METHACRYLATE
Biocompatible Materials
ADHESION
Microbiology
Mammalian cell
Cell Line, Tumor
Staphylococcus epidermidis
AUREUS
Humans
Polymethyl Methacrylate
HUMAN-ENDOTHELIAL CELLS
Osteosarcoma
TISSUE INTEGRATION
Chemistry
POLYMER SURFACES
Biofilm
Implant failure
Biomaterial
Adhesion
Prostheses and Implants
Contamination
In vitro
SMALL-COLONY VARIANTS
INFECTIONS
Biofilms
BACTERIA
Implant
STAPHYLOCOCCUS-EPIDERMIDIS
Subjects
Details
- ISSN :
- 14652080 and 13500872
- Volume :
- 156
- Issue :
- Pt 10
- Database :
- OpenAIRE
- Journal :
- Microbiology (Reading, England)
- Accession number :
- edsair.doi.dedup.....785f0b0d88044297cb6dd9010b895e9e