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HPV DNA and p53 alterations in oropharyngeal carcinomas
- Source :
- Virchows Archiv : an international journal of pathology. 427(2)
- Publication Year :
- 1995
-
Abstract
- We have examined a series of 37 oropharyngeal squamous cell carcinomas for the presence of HPV 6/11, 16, and 18 DNA by polymerase chain reaction (PCR)/Southern blotting and for p53 alterations by immunohistochemistry and mutation screening with temperature gradient gel electrophoresis (TGGE). HPV sequences were found in a total of 26 of 37 cancers (70.3%), most frequently HPV 16 (20/37) followed by HPV 18 (11/37). Double infections with HPV 16 and 18 were present in 5 tumours. p53 accumulation was detectable immunohistochemically in 21 of 37 carcinomas (56.8%). There were remarkable differences in the distribution of immunoreactive tumour cells in relation to the tumour grade. A mutation screening for p53 by TGGE, directed to the amplified exons 5-8, revealed p53 mutations in 14 of 37 carcinomas (37.8%). Mutations in two different exons were present in 3 tumours, 11 tumours being hit once. Exon 7 was mutated in 6 carcinomas, exons 5 and 8 in 4 cases, and exon 6 in 3 cases. When grouping the tumours with p53 mutation according to their HPV state, HPV-positive cases showed slightly more mutations (11/26) than HPV-negative cases (3/11). Only 5 of 37 carcinomas (13.5%) contained neither HPV DNA nor p53 alterations. Our results indicate that high-risk HPV and p53 mutations frequently coexist in oropharyngeal carcinomas, in contrast to genital tumours, notably carcinomas of the cervix uteri. This may reflect different pathways in carcinogenesis in squamous cell epithelium from different sites.
- Subjects :
- Adult
Male
Biology
medicine.disease_cause
Pathology and Forensic Medicine
law.invention
Exon
law
medicine
Humans
Papillomaviridae
Molecular Biology
Polymerase chain reaction
Southern blot
Aged
Aged, 80 and over
Mutation
Cell Biology
General Medicine
Middle Aged
biology.organism_classification
Genes, p53
Molecular biology
Oropharyngeal Neoplasms
Oropharyngeal Neoplasm
Oropharyngeal Carcinoma
DNA, Viral
Carcinoma, Squamous Cell
Female
Tumor Suppressor Protein p53
Carcinogenesis
Subjects
Details
- ISSN :
- 09456317
- Volume :
- 427
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Virchows Archiv : an international journal of pathology
- Accession number :
- edsair.doi.dedup.....78a5413cfa9e4d546e484c3f69a7be65