Supplemental Methods and Figures 1-5 from Relative Target Affinities of T-Cell–Dependent Bispecific Antibodies Determine Biodistribution in a Solid Tumor Mouse Model

Supplemental Methods, followed by Supplemental Figures S1, S2, S3, S4 and S5. Supplementary Fig. S1 In vitro binding of TDBs to HER2 and CD3 expressing cell lines. Supplementary Fig. S2 HER2 expression in model cell lines in vitro and tumors in vivo. Supplementary Fig. S3 HER2, CD3� and MECA-32 staining and vascular characterization of HER2 positive and negative tumors. Supplementary Fig. S4 T cell infiltration and CD3 expression in murine CT26 and CT26-HER2 tumors. Supplementary Fig. S5 Pharmacokinetic data from whole blood and cell pellet fractions