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Characterization of a novel Cotesia vestalis polydnavirus (CvBV) gene containing a ser-rich motif expressed in Plutella xylostella larvae
- Source :
- BMB Reports. 41:587-592
- Publication Year :
- 2008
- Publisher :
- Korean Society for Biochemistry and Molecular Biology - BMB Reports, 2008.
-
Abstract
- Cotesia vestalis is an endoparasitoid of Plutella xylostella larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we characterize the gene, CvBV3307, and its products. CvBV3307 is located on segment S33 of the CvBV genome, is 517 bp, and encodes a putative protein of 122 amino acids, including a serine-rich region. The expression pattern of CvBV3307 in parasitized larvae and the subcellular localization of CvBV3307 only in granulocytes indicated that it might be involved in early protection of parasitoid eggs from host cellular encapsulation and in manipulating the hormone titer and developmental rhythm of host larvae. Western blot analysis showed that the size of the immunoreactive protein (about 55 kDa) in parasitized hosts at 48 hours post parasitization (h p.p.) is much larger than the predicted molecular weight of 13.6 kDa, which suggests that CvBV3307 undergoes extensive post-translational modification in hosts.
- Subjects :
- Genes, Viral
Molecular Sequence Data
Wasps
Gene Expression
Moths
Biology
Bioinformatics
Biochemistry
Parasitoid
Viral Proteins
Western blot
Gene expression
medicine
Animals
Amino Acid Sequence
RNA, Messenger
Cotesia vestalis
Molecular Biology
Gene
Peptide sequence
Base Sequence
Sequence Homology, Amino Acid
medicine.diagnostic_test
Polydnavirus
fungi
Plutella
General Medicine
biology.organism_classification
Molecular biology
Retraction
Molecular Weight
Polydnaviridae
Larva
DNA, Viral
RNA, Viral
Protein Processing, Post-Translational
Subjects
Details
- ISSN :
- 19766696
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....78b4059770c0fda7ef502a331cc49994