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Stimulatory effects of benzene on rabbit liver and kidney microsomal cytochrome P-450 dependent drug metabolizing enzymes
- Source :
- Archives of Toxicology. 65:186-190
- Publication Year :
- 1991
- Publisher :
- Springer Science and Business Media LLC, 1991.
-
Abstract
- Treatment of rabbits with benzene (880 mg/kg/day), s.c. for 3 consecutive days, caused 3.8- and 5.7-fold increases in aniline 4-hydroxylation rates of liver and kidney microsomes, respectively. Benzene treatment markedly enhanced hydroxylation rats of p-nitrophenol by liver and kidney by 7.2- and 4.2-fold, respectively. Both of these enzymes are associated with cytochrome P-450 LM3a. In contrast, the activity of benzphetamine N-demethylase, associated with P-450 LM2, was not altered significantly in either liver or kidney microsomes. Although the total cytochrome P-450 contents of liver and kidney microsomes were not altered significantly by the benzene treatment, in the case of liver microsomes, formation of a new cytochrome P-450 with an apparent Mr of 51,400 was observed on SDS-PAGE. On the other hand, in the kidney microsomes, the intensity of the bands corresponding to approximate Mr of 50,000 and 51,400 was markedly increased. The results of the present work, in combination with those of the previous work (Arinç et al. 1988), indicate the existence of tissue specificity in the induction of rabbit P-450 isozyme by benzene.
- Subjects :
- Male
medicine.medical_specialty
Cytochrome
Health, Toxicology and Mutagenesis
Kidney
Toxicology
Isozyme
Hydroxylation
chemistry.chemical_compound
Cytochrome P-450 Enzyme System
Microsomes
Internal medicine
medicine
Animals
Lung
chemistry.chemical_classification
biology
Chemistry
Cytochrome P450
Benzene
General Medicine
Endocrinology
medicine.anatomical_structure
Enzyme
Pharmaceutical Preparations
Microsomes, Liver
biology.protein
Microsome
Electrophoresis, Polyacrylamide Gel
Rabbits
Benzphetamine
medicine.drug
Subjects
Details
- ISSN :
- 14320738 and 03405761
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Archives of Toxicology
- Accession number :
- edsair.doi.dedup.....78e57d19d3b4ca42af9984fd4f029ebd