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Aromatase (P450arom) and 11beta-hydroxylase (P45011beta) genes are differentially expressed during the sex change process of the protogynous rice field eel, Monopterus albus
- Source :
- Fish Physiology and Biochemistry, Fish Physiology and Biochemistry, Springer Verlag, 2009, 35 (3), pp.511-518. ⟨10.1007/s10695-008-9255-9⟩
- Publication Year :
- 2008
-
Abstract
- International audience; Steroids are known to play a crucial role in gonadal sex differentiation in many non-mammalian vertebrates, but also in the gonadal sex change of hermaphroditic teleosts. We investigated the expression of two genes encoding key steroidogenic enzymes, i.e., cytochrome P450 aromatase (P450arom) and cytochrome P45011 beta-hydroxylase (P45011 beta), during the sex change of the protogynous rice field eel, Monopterus albus. Using RT-PCR with degenerate primers, we cloned rice field eel homologous fragments for both genes (rcP450arom and rcP45011 beta) as indicated by the high level of homology with P450arom and P45011 beta sequences from various vertebrates. Gonadal expression of rcP450arom and rcP45011 beta mRNA levels were then assessed during the sex change by semi-quantitative RT-PCR and a real-time RT-PCR. rcP450arom was predominantly expressed in ovary, much less in ovotestis, and barely in testis. Conversely, P45011 beta was markedly up-regulated at the onset of testicular development. These findings underline that regulation of steroidogenesis is an important process in the sex change of protogynous rice field eel, and they clearly indicate that the concomitant down-regulation of P450arom and up-regulation of P45011 beta are of pivotal importance to the sex change of this species.
- Subjects :
- Male
Sex Differentiation
Physiology
synbranchidae
Monopterus albus
Biochemistry
Homology (biology)
Testis
sex change
Cluster Analysis
Aromatase
Cloning, Molecular
Phylogeny
0303 health sciences
biology
Reverse Transcriptase Polymerase Chain Reaction
[SDV.BA]Life Sciences [q-bio]/Animal biology
rice field eel
04 agricultural and veterinary sciences
General Medicine
Smegmamorpha
Female
Monopterus
medicine.medical_specialty
endocrine system
Molecular Sequence Data
Aquatic Science
Gene Expression Regulation, Enzymologic
03 medical and health sciences
Sex change
Internal medicine
medicine
Animals
Gene
030304 developmental biology
DNA Primers
P450 11β-hydroxylase
Sexual differentiation
Ovotestis
Base Sequence
Ovary
Cytochrome P450
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
Sequence Analysis, DNA
biology.organism_classification
Endocrinology
P450 aromatase
040102 fisheries
biology.protein
0401 agriculture, forestry, and fisheries
Steroid 11-beta-Hydroxylase
sense organs
Subjects
Details
- ISSN :
- 15735168 and 09201742
- Volume :
- 35
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Fish physiology and biochemistry
- Accession number :
- edsair.doi.dedup.....79110c091e04d94e9afe348b591dba16