Back to Search
Start Over
Intramuscular gene transfer of interleukin-10 cDNA reduces atherosclerosis in apolipoprotein E-knockout mice
- Source :
- Atherosclerosis. 172(1)
- Publication Year :
- 2004
-
Abstract
- Atherosclerosis has a close relationship to inflammation, particularly T helper type 1 lymphocyte (Th1) response. Interleukin-10 (IL-10), is thought to suppress Th1 response. To target therapeutic strategy for atherosclerosis, we tested whether IL-10 gene transfer suppresses atherosclerosis in apolipoprotein E-knockout (apoE-KO) mice. Four-week-old apoE-KO mice were divided into two groups and either murine IL-10 cDNA plasmid or empty control vector was transferred to the femoral muscle with the use of Hemagglutinating virus of Japan (HVJ)-liposome. At 1 week after transfection, high cholesterol diet was started and continued for 8 weeks. After euthanasia, histological studies of atherosclerotic lesions and quantitative RT-PCR for Th1 cytokines (IL-12 and IFN-gamma) in spleens were performed. IL-10 cDNA gene transfer to the muscle increased plasma IL-10 levels and depressed expression of Th1 cytokines without changing plasma cholesterol levels. IL-10 gene transfer significantly reduced the atherosclerotic plaque area and the macrophage infiltrated area. IL-12 and IFN-gamma mRNA expressions in spleens and plasma IFN-gamma levels were decreased by IL-10 gene transfer. Therefore, IL-10 gene transfer changed the Th1 response and suppressed atherosclerotic lesion formation in apoE-KO mice. IL-10 could be a new target as a therapeutic tool for the treatment of atherosclerosis.
- Subjects :
- Apolipoprotein E
Male
medicine.medical_specialty
DNA, Complementary
Apolipoprotein B
Arteriosclerosis
medicine.medical_treatment
Genetic Vectors
Inflammation
Sendai virus
chemistry.chemical_compound
Interferon-gamma
Mice
Apolipoproteins E
Internal medicine
medicine
Animals
RNA, Messenger
Mice, Knockout
biology
Cholesterol
Reverse Transcriptase Polymerase Chain Reaction
Gene Transfer Techniques
Transfection
Genetic Therapy
Interleukin-12
Interleukin-10
Interleukin 10
Cytokine
Endocrinology
chemistry
Knockout mouse
Immunology
Liposomes
biology.protein
medicine.symptom
Cardiology and Cardiovascular Medicine
Plasmids
Subjects
Details
- ISSN :
- 00219150
- Volume :
- 172
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Atherosclerosis
- Accession number :
- edsair.doi.dedup.....791cd0e2f7216843f4e4efb31ea74e0d