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Characterization of Ferric Chloride-Induced Arterial Thrombosis Model of Mice and the Role of Red Blood Cells in Thrombosis Acceleration
- Source :
- Yonsei Medical Journal
- Publication Year :
- 2021
-
Abstract
- PURPOSE The ferric chloride (FeCl3)-induced thrombosis model is widely used for thrombosis research. However, it lacks standardization with uncertainty in the exact mechanism of thrombosis. This study aimed to characterize thrombus formation in a mouse model. MATERIALS AND METHODS We investigated thrombus formation and stability using various FeCl3 concentrations (10%, 20%, 30%, 40%, and 50%, w/v) in carotid arteries of the Institute of Cancer Research (ICR) and C57BL/6N mice using the FeCl3-induced thrombosis model. We also investigated thrombus histopathology using immunohistochemistry and electron microscopy. RESULTS Higher FeCl3 concentrations induced dose-dependent, faster, larger, and more stable thrombus formation in both strains of mice. However, the ICR mice showed better dose-responses in thrombus formation and stability compared to the C57BL/6N mice. Thrombi were fibrin- and platelet-rich without significant changes across FeCl3 concentrations. However, the content of red blood cells (RBCs) increased with increasing FeCl3 concentrations (p for trend
- Subjects :
- medicine.medical_specialty
Pathology
Erythrocytes
Acceleration
experimental animal models
Ferric Compounds
Fibrin
law.invention
Mice
Chlorides
law
medicine
Animals
Platelet
Platelet activation
cardiovascular diseases
Thrombus
platelet
biology
Chemistry
Neurology & Neurosciences
Thrombosis
General Medicine
medicine.disease
Mice, Inbred C57BL
biology.protein
Immunohistochemistry
Histopathology
Original Article
Electron microscope
circulatory and respiratory physiology
red blood cells
Subjects
Details
- ISSN :
- 19762437
- Volume :
- 62
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Yonsei medical journal
- Accession number :
- edsair.doi.dedup.....791eca8976ace3e9254e5282ae31c3a5