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Sea urchin embryos exposed to cadmium as an experimental model for studying the relationship between autophagy and apoptosis
Sea urchin embryos exposed to cadmium as an experimental model for studying the relationship between autophagy and apoptosis
- Source :
- Marine Environmental Research. 93:47-55
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- The sea urchin embryo is a suitable model that offers an excellent opportunity to investigate different defence strategies activated in stress conditions. We previously showed that cadmium accumulates in a dose- and time-dependent manner into embryonic cells, activating different stress and defence mechanisms, including the synthesis of HSPs and the onset of apoptosis and/or autophagy. In this paper we investigated the functional relationship between autophagy and apoptosis, evaluating apoptosis signals in cadmium-exposed Paracentrotus lividus embryos with inhibited autophagy. We found that the inhibition of autophagy produced the concurrent reduction of apoptosis, suggesting that the two phenomena are functionally related. Considering the catabolic role of autophagy, an energetic hypothesis to explain the relationship was evaluated. Using a substrate for ATP production, we found that apoptosis, assessed by TUNEL and cleaved caspase-3 immunocytochemistry, was substantially restored in cadmium-treated embryos where autophagy was inhibited by 3-Methyladenine. On the basis of these results, we propose that, autophagy could play a crucial role in stress response of this organism because autophagy could energetically contribute to apoptotic execution through its catabolic role.
- Subjects :
- Programmed cell death
Embryo, Nonmammalian
Immunocytochemistry
Apoptosis
Aquatic Science
Biology
Oceanography
Apoptosis, Autophagy, Stress, Cadmium, Sea urchin Embryo
Paracentrotus lividus
Autophagy
Animals
Settore BIO/06 - Anatomia Comparata E Citologia
TUNEL assay
Caspase 3
Embryo
General Medicine
biology.organism_classification
Pollution
Embryonic stem cell
Cell biology
Models, Animal
Paracentrotus
Water Pollutants, Chemical
Cadmium
Subjects
Details
- ISSN :
- 01411136
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Marine Environmental Research
- Accession number :
- edsair.doi.dedup.....792c95eaeb32eeb5ec29c4426038cb2e
- Full Text :
- https://doi.org/10.1016/j.marenvres.2013.06.001