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Osteogenic-differentiated Mesenchymal Stem Cell-secreted Extracellular Matrix as a Bone Morphogenetic Protein-2 delivery system for ectopic bone formation
- Source :
- ACTA BIOMATERIALIA, ACTA BIOMATERIALIA, 2020, 116, pp.186-200. ⟨10.1016/j.actbio.2020.09.003⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- International audience; While human bone morphogenetic protein-2 (BMP-2) is a promising growth factor for bone regeneration, a major challenge in biomedical applications is finding an optimal carrier for its delivery at the site of injury. Because of their natural affinities for growth factors (including BMP-2) as well as their role in instructing cell function, cultured cell-derived extracellular matrices (ECM) are of special interest. We hereby hypothesized that a “bony matrix” containing mineralized, osteogenic ECM is a potential efficacious carrier of BMP-2 for promoting bone formation and, therefore, compared the efficacy of the decellularized ECM derived from osteogenic-differentiated human mesenchymal stem cells (hMSCs) to the one obtained from ECM from undifferentiated hMSCs. Our results provided evidence that both ECMs can bind BMP-2 and promote bone formation when implanted ectopically in mice. The osteoinductive potential of BMP-2, however, was greater when loaded within an osteogenic MSC-derived ECM; this outcome was correlated with higher sequestration capacity of BMP-2 over time in vivo. Interestingly, although the BMP-2 mainly bound onto the mineral crystals contained within the osteogenic MSC derived-ECM, these mineral components were not involved in the observed higher osteoinductivity, suggesting that the organic components were the critical components for the matrix efficacy as BMP-2 carrier.
- Subjects :
- Bone Regeneration
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
medicine.medical_treatment
[SDV]Life Sciences [q-bio]
0206 medical engineering
Biomedical Engineering
Bone Morphogenetic Protein 2
02 engineering and technology
Biochemistry
Bone morphogenetic protein 2
Biomaterials
Extracellular matrix
Mice
Osteogenesis
In vivo
medicine
Extracellular
Animals
Bone regeneration
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
Molecular Biology
Cells, Cultured
Decellularization
Chemistry
Growth factor
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
021001 nanoscience & nanotechnology
020601 biomedical engineering
dissemin
Extracellular Matrix
Cell biology
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
[SDV] Life Sciences [q-bio]
[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials
0210 nano-technology
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- ACTA BIOMATERIALIA, ACTA BIOMATERIALIA, 2020, 116, pp.186-200. ⟨10.1016/j.actbio.2020.09.003⟩
- Accession number :
- edsair.doi.dedup.....7950a33dacd236a11e04db2b7227b386