Back to Search Start Over

Osteogenic-differentiated Mesenchymal Stem Cell-secreted Extracellular Matrix as a Bone Morphogenetic Protein-2 delivery system for ectopic bone formation

Authors :
Esther Potier
Niclas Setterblad
M. Bensidhoum
Delphine Logeart-Avramoglou
Nathanaël Larochette
Hervé Petite
Hanane El-Hafci
Biologie, Bioingénierie et Bioimagerie Ostéo-articulaires (B3OA (UMR_7052))
Institut National de la Santé et de la Recherche Médicale (INSERM)-École nationale vétérinaire d'Alfort (ENVA)-Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS)
Institut de Recherche Saint-Louis - Hématologie Immunologie Oncologie (Département de recherche de l’UFR de médecine
ex- Institut Universitaire Hématologie-IUH) (IRSL)
Université de Paris (UP)
Biologie, Bioingénierie et Bioimagerie Ostéo-articulaires (B3OA)
École nationale vétérinaire - Alfort (ENVA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)
Université Paris Cité (UPCité)
Logeart-Avramoglou, Delphine
Source :
ACTA BIOMATERIALIA, ACTA BIOMATERIALIA, 2020, 116, pp.186-200. ⟨10.1016/j.actbio.2020.09.003⟩
Publication Year :
2020
Publisher :
HAL CCSD, 2020.

Abstract

International audience; While human bone morphogenetic protein-2 (BMP-2) is a promising growth factor for bone regeneration, a major challenge in biomedical applications is finding an optimal carrier for its delivery at the site of injury. Because of their natural affinities for growth factors (including BMP-2) as well as their role in instructing cell function, cultured cell-derived extracellular matrices (ECM) are of special interest. We hereby hypothesized that a “bony matrix” containing mineralized, osteogenic ECM is a potential efficacious carrier of BMP-2 for promoting bone formation and, therefore, compared the efficacy of the decellularized ECM derived from osteogenic-differentiated human mesenchymal stem cells (hMSCs) to the one obtained from ECM from undifferentiated hMSCs. Our results provided evidence that both ECMs can bind BMP-2 and promote bone formation when implanted ectopically in mice. The osteoinductive potential of BMP-2, however, was greater when loaded within an osteogenic MSC-derived ECM; this outcome was correlated with higher sequestration capacity of BMP-2 over time in vivo. Interestingly, although the BMP-2 mainly bound onto the mineral crystals contained within the osteogenic MSC derived-ECM, these mineral components were not involved in the observed higher osteoinductivity, suggesting that the organic components were the critical components for the matrix efficacy as BMP-2 carrier.

Details

Language :
English
Database :
OpenAIRE
Journal :
ACTA BIOMATERIALIA, ACTA BIOMATERIALIA, 2020, 116, pp.186-200. ⟨10.1016/j.actbio.2020.09.003⟩
Accession number :
edsair.doi.dedup.....7950a33dacd236a11e04db2b7227b386