Additional file 1 of Ion-Channel modulator TH1177 reduces glomerular injury and serum creatinine in chronic mesangial proliferative disease in rats

Additional file 1: Supplementary Fig. 1. PAS histology of the chronic Thy1 model at days 7, 14, 28 and 42. There is progressive glomerular hypercellularity as quantified in the lower panel. Supplementary Fig. 2. ED1 stain for monocyte/macrophages in the chronic Thy1 model at days 7,14, 28 and 42. Scale bars are 100 μm. There is an early inflammatory increase in ED1 positive cells at day 7. This tends to reduce with few ED1 positive cells in the glomeruli at day 42 of the model. Supplementary Fig. 3. Ki-67 stain of the Chronic Thy1 model as an estimate of cellular proliferation with quantification in the chart at the right. Scale bars are 100 μm. The disease model shows an increase in Ki67 stain at day 28 with later resolution so that there is no difference between disease and vehicle controls by day 42. Supplementary Fig. 4 Plot of serum creatinine against body weight at Day 42 in the therapeutic experiment on the chronic Thy1 model. There was no significant correlation seen.